We narrowed to 13,020 results for: cDNA

1. pcDNA5/FRT/TO-EGFP-RIP2

   Plasmid

   #131201

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

   Insert

   RIPK2 (RIPK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 IGF1R-BirA(R118G)-HA

   Plasmid

   #109232

   Purpose

   This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

   Depositor

   Victor Dzau

   Article

   Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   BirA(R118G) (highly promiscuous form) and HA

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AU1-mTOR-S2215Y

   Plasmid

   #26037

   Depositor

   Fuyuhiko Tamanoi

   Article

   Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

   Insert

   mammalian target of rapamycin-S2215Y (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   S2215Y

   Promoter

   Available since

   Sept. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-CMV-mCherry-hPMCA2w/b

   Plasmid

   #111570

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Myc-His beta4

   Plasmid

   #16039

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Dest47- C11orf83-GFP

   Plasmid

   #65844

   Purpose

   Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   UQCC3 WT (UQCC3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3. Flag-CSL R192H

   Plasmid

   #167005

   Purpose

   Mammalian expression of CSL R192H

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R192H

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1D V5-His-TOPO-HuR.wt

   Plasmid

   #110417

   Purpose

   Mammalian expression of HuR (wild type)

   Depositor

   Sandra Martha Gomes Dias

   Insert

   HuR (ELAVL1) (ELAVL1 Human)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/avitag-apex2-v5-fkbp8

   Plasmid

   #154460

   Purpose

   expresses avitag-apex2-v5-fkbp8/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   avitag-apex2-v5-fkbp8 (FKBP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F, T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1- dCas9-2xNLS-EGFP

    Plasmid

    #74710

    Purpose

    Expresses dCas9-2xNLS-EGFP in mammalian cells

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    dCas9-2xNLS-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-OTUB1 C91S-pcDNA3.1

    Plasmid

    #118210

    Purpose

    Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-TAT-1-101-FLAG

    Plasmid

    #35981

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1 101 amino acids

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CibN-dCas9-CibN

    Plasmid

    #60553

    Purpose

    Expresses CibN-dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA GASTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71295

    Purpose

    Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    GASTM mutated glycosylation motif, HA, Lamp2 sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Mar 18. ():.

    Insert

    opto-a1AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

    Plasmid

    #115788

    Purpose

    Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    contains silent mutation that creates an internal…

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid

    #223952

    Purpose

    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-NES-PhoCl2f-mCherry

    Plasmid

    #164048

    Purpose

    Optogenetic control of protein localization by PhoCl2f

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2f-mCherry
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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