We narrowed to 13,291 results for: cDNA

1. pcDNA3.1/hChR2(H134R)-mCherry

   Plasmid

   #20938

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA FLAG TORC1 (1-634)

   Plasmid

   #25718

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

   Insert

   TORC1 (1-634) (CRTC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Myc-NLRP3(R260W)

   Plasmid

   #73956

   Purpose

   Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

   Insert

   NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   R260W

   Promoter

   CMV iE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 Flag MKK7B2Jnk1a1(APF)

   Plasmid

   #19730

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   replaced dual phosporylation motif Thr(1959)-Pro-…

   Promoter

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4TO-24xGCN4_v4-BFP-24xPP7

   Plasmid

   #74929

   Purpose

   Translation reporter

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   24xGCN4_v4-BFP-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4 human alpha4 full

   Plasmid

   #221397

   Purpose

   Mammalian expression of human integrin alpha4 full length

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin alpha4 full length (ITGA4 Human)
   

   Use

   Tags

   Strep twin

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-sACE2v2.4(732)-IgG1

   Plasmid

   #154106

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available Since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNAintron-CHIKV-Structural polyprotein

   Plasmid

   #215698

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-E2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-FLAG-MTOR-R2505P

   Plasmid

   #69015

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-R2505P (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Arginine 2505 to Proline

   Promoter

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid

    #141183

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Delta(Pro-MP)ADAM10-HA

    Plasmid

    #65107

    Purpose

    Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 19-455

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-H2B-AID-EYFP

    Plasmid

    #47329

    Purpose

    for plasmid integration using the Flp-In system. AID at C-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    AID and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1D V5-His-TOPO-KGA.wt

    Plasmid

    #110332

    Purpose

    Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

    Depositor

    Sandra Martha Gomes Dias

    Article

    Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid

    #236097

    Purpose

    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 HA-PHLPP1 full length

    Plasmid

    #37100

    Purpose

    Expresses HA tagged, full-length human PHLPP1 in mammalian cells

    Depositor

    Alexandra Newton

    Article

    Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

    Insert

    PHLPP1 (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187922

    Purpose

    Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Use

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-puro-eGFP-HEC1

    Plasmid

    #114022

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-EGFP-Rac1(Q61L)

    Plasmid

    #13720

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    EGFP-Rac1(Q61L) (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-neo-cterminal-3HA

    Plasmid

    #102643

    Purpose

    To generate three copies of the HA epitope tag on cDNAs.

    Depositor

    Michael E. Wright

    Type

    Empty backbone

    Use

    Tags

    3x HA (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-His:dCas9:nls:10P3

    Plasmid

    #135982

    Purpose

    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    dCas9 fused to 10 copies of the P3 coiled-coil
    

    Use

    CRISPR

    Tags

    His

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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