We narrowed to 7,654 results for: pac

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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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HERV-Kcon-RT for Split-PE (pJUL3120)

Plasmid

#190107

Purpose

Expresses HERV-Kcon RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

HERV-Kcon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pNB22

Plasmid

#103059

Purpose

medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp

Depositor

Dirk Bumann

Article

Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

Insert

GFP-OVA

Tags

none

Expression

Bacterial

Promoter

hmpAp

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa-VPR mini.

Plasmid

#99685

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps_LacZα_gIII

Plasmid

#190435

Purpose

M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

M13 packaging signal
M13 gIII
LacZα

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV8 L1L2

Plasmid

#52598

Purpose

Expresses HPV8 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV8 L1
HPV8 L2

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMP4639

Plasmid

#107771

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pME6010

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV38 L1L2

Plasmid

#52594

Purpose

Expresses HPV38 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV38 L1
HPV38 L2

Expression

Mammalian

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His Snk/Plk2

Plasmid

#16015

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Tags

V5-His

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pC018 - LshCas13a from Leptotrichia shahii

Plasmid

#91908

Purpose

Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LshCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMA2640

Plasmid

#25434

Purpose

Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

reverse tetracycline-regulated transactivator Advanced

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-His6

Plasmid

#182386

Purpose

Bacterial expression of Vesicle Nucleating peptide

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV18 L1L2

Plasmid

#52592

Purpose

Expresses HPV18 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV18 L1
HPV18 L2

Expression

Mammalian

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MP6-SlugABEN-738

Plasmid

#214348

Purpose

Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE

Expression

Bacterial

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTPH412

Plasmid

#185724

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-gp41-8N

Use

CRISPR

Expression

Bacterial

Mutation

ecTadA(8e) R26G

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9 nickase for Split-PE (pJUL2497)

Plasmid

#190104

Purpose

Expresses nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Tags

XTEN-bpNLS and bpNLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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