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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Publication

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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1072 pBabe puroL myr Akt K179M T308A S473A

Plasmid

#9015

Depositor

William Sellers

Publication

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

K179M T308A S473A

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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hAAVS1 TALEN Right

Plasmid

#52342

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Right

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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hAAVS1 TALEN Left

Plasmid

#52341

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Left

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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3XFlagNICD4

Plasmid

#20186

Depositor

Raphael Kopan

Publication

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Notch 4 Intracellular Domain (Notch4 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 4 Intracellular Domain (Val…

Promoter

CMV

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Flag TSC2 wt

Plasmid

#12132

Depositor

Cheryl Walker

Publication

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Publication

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Publication

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-HA-RIPK1

Plasmid

#78834

Purpose

To overexpress RIPK1 in Mammalian Cell

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Publication

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJMK001

Plasmid

#33780

Depositor

Adam Cohen

Publication

Kralj et al Science. 2011 Jul 15;333(6040):345-8.

Insert

Proteorhodopsin Optical Proton Sensor

Expression

Bacterial

Mutation

D97N

Promoter

AraBAD

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFastBac Dual His6 TEV LIC cloning vector (5B)

Plasmid

#30122

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-TEV

Expression

Insect

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBABE-hLin28B

Plasmid

#26358

Depositor

George Daley

Publication

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog B (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHes1(2.5k)-luc

Plasmid

#43806

Depositor

Ryoichiro Kageyama

Publication

Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hes1 Promoter (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter

Promoter

Hes1 Promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLKO.1 puro CXCR4 siRNA-1

Plasmid

#12271

Depositor

Bob Weinberg

Publication

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Publication

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KSRP (KHSRP Human)

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HBM-Luc

Plasmid

#35155

Depositor

Linda Penn

Publication

Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Myc promoter (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains 2.5 kb of the c-myc minimal promoter

Promoter

c-myc

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-Rab5CA (Q79L)

Plasmid

#35140

Depositor

Sergio Grinstein

Publication

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pBAD/HisB-PAmCherry1

Plasmid

#31931

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

His

Expression

Bacterial

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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