20,093 results

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Article

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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EX-HA-FRB-Cb5_pLX304

Plasmid

#120915

Purpose

Lentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

EX-HA-FRB-Cb5

Use

Lentiviral

Expression

Mammalian

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTA7001-DEST

Plasmid

#71745

Purpose

GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Expression

Plant

Promoter

GAL4

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-Cre.zf1

Plasmid

#61391

Purpose

mRNA synthesis of Cre recombinase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cre.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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phDLX1-N174

Plasmid

#60859

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX1 (DLX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-luciferase

Plasmid

#55764

Purpose

luciferase reporter for in vivo bioluminescence imaging

Depositor

Snorri Thorgeirsson

Article

Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

Insert

CMV enhancer/chicken beta actin promoter

Expression

Mammalian

Promoter

CMV enhancer/chicken beta actin promoter

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-lck-GCaMP6f

Plasmid

#52924

Purpose

Fluorescent reporter for calcium signaling

Depositor

Baljit Khakh

Has Service

AAV5

Insert

lck-GCaMP6f

Use

AAV

Tags

6xHis

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-BbTagBY

Plasmid

#45185

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

Plasmid

#40640

Depositor

Brenda Lilly

Article

Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

Insert

NICD3 (NOTCH3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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