We narrowed to 18,277 results for: Met;

1. pmScarlet-i_Giantin_C1

   Plasmid

   #85050

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1_VNp-mNeongreen-Etanercept

   Plasmid

   #182421

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-Etanercept (TNFRSF1B Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB2-mScarlet-I

   Plasmid

   #104007

   Purpose

   Plasmid encoding mScarlet-I

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mScarlet-I
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111545

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChrimson_tdtomato_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-P3-IgKL-mNG

   Plasmid

   #183465

   Purpose

   To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   IgKL-mNeonGreen
   

   Use

   AAV

   Tags

   IgKL secretory signal

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

   Plasmid

   #154139

   Purpose

   Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
   

   Use

   Tags

   3x FLAG and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

   Plasmid

   #104563

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1 (D832A)
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS15 (hANGPTL3-Strep)

    Plasmid

    #109112

    Purpose

    Expresses StrepII-tagged human ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (ANGPTL3 Human)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTXB1-pA-M.EcoGII

    Plasmid

    #192873

    Purpose

    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

    Depositor

    Chong Tang

    Article

    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

    Insert

    pA-M.EcoGII
    

    Use

    Tags

    3X Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-mDia1(FH1FH2DAD)-His

    Plasmid

    #85822

    Purpose

    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

    Depositor

    Antoine Jégou, Guillaume Romet-Lemonne

    Article

    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

    Insert

    Protein diaphanous homolog 1 (Diaph1 Mouse)
    

    Use

    Tags

    8*His and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-PTBP1CCtoSS

    Plasmid

    #89157

    Purpose

    Recombinant expression of PTBP1 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid

    #84239

    Purpose

    Expresses ABA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MICU-APEX2

    Plasmid

    #79057

    Purpose

    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1_SPTLC1_WT

    Plasmid

    #181920

    Purpose

    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

    Depositor

    Christian Metallo

    Article

    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

    Insert

    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    

    Use

    Lentiviral; Mammalian expression, , doxycycline i…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet-H_Giantin_C1

    Plasmid

    #85049

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEB2-mScarlet

    Plasmid

    #104006

    Purpose

    Plasmid encoding mScarlet

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge