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pBbB19k-GFP

Plasmid

#126135

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pALOD4 non-binding mutant

Plasmid

#111027

Purpose

Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A, G501A, T502A, T503A, L5…

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHES795

Plasmid

#87943

Purpose

Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

Zif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)

Expression

Yeast

Promoter

ADH1 (crippled)

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-Vinculin

Plasmid

#80024

Purpose

Mammalian expression of mCherry-vinculin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pFM689

Plasmid

#74635

Purpose

alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Inserts

Expression

Yeast

Promoter

none

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pHnCBS1D

Plasmid

#52065

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D

Expression

Bacterial

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

Plasmid

#192184

Purpose

Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

Plasmid

#192183

Purpose

Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP15_nostop

Plasmid

#149317

Purpose

Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP14_nostop

Plasmid

#149316

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP7_nostop

Plasmid

#149310

Purpose

Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

Plasmid

#200641

Purpose

Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

Depositor

Justin English, Fleur Ferguson

Article

Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

Insert

miniTurboID-6xGGSG linker-FKBPF36V-2xHA

Tags

HA-tag

Expression

Mammalian

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NINJ1

Plasmid

#201180

Purpose

Transient expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPLV_C1

Plasmid

#186415

Purpose

Endogenous murine RNase inhibitor production for cell-free lysate

Depositor

Jerome Bonnet

Article

Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

Insert

Murine RNase Inhibitor

Use

Synthetic Biology

Promoter

T7

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJL056

Plasmid

#177168

Purpose

Expresses wildtype MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 mutant reporter

Plasmid

#91703

Purpose

lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 mutant promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant E-boxes

Promoter

CDH1 mutant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Tags

myc tag

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-CRY2-OCRL

Plasmid

#79565

Purpose

expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

Depositor

Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

OCRL (OCRL Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539

Promoter

CMV

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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