19,635 results

1. pUSE-Src-YF-UniRapR-mCerulean-myc

   Plasmid

   #45381

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   Src-YF-UniRapR-mCerulean-myc
   

   Tags

   Myc, Myr, and mCerulean

   Expression

   Mammalian

   Mutation

   changed mSrc Tyrosine 529 to Phenylalanine

   Promoter

   CMV

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. HIF-TRE-mStrawberry reporter

   Plasmid

   #158681

   Purpose

   This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2

   Plasmid

   #60625

   Purpose

   Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 mouse MyoD
   

   Use

   Lentiviral

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFOK

   Plasmid

   #166651

   Purpose

   Suicide vector for gene manipulation

   Depositor

   Dirk Bumann

   Article

   Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

   Insert

   I-sceI, sacB
   

   Use

   Synthetic Biology

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX307-CRBN-3F-BirA

   Plasmid

   #220146

   Purpose

   Expresses CRBN-BirA for CRBN substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   Cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b His6-FKBPF36V

   Plasmid

   #73180

   Purpose

   Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

   Depositor

   Thomas Wandless

   Article

   Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

   Insert

   FKBP(F36V) (FKBP1A Human)
   

   Tags

   His 6x

   Expression

   Bacterial

   Mutation

   F36V

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pLVX-Tight-Puro_Sox10

   Plasmid

   #46536

   Purpose

   A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Sox10 (Sox10 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-hsyn-GRAB_ATP1.0

   Plasmid

   #167577

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3/UBC9

    Plasmid

    #20082

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

    Insert

    UBC9 (UBE2I Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX311-Cas9

    Plasmid

    #118018

    Purpose

    Constitutive expression of Cas9

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Cas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TLR4-YFP

    Plasmid

    #13018

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 4 (TLR4 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Tet1

    Plasmid

    #60938

    Purpose

    Expresses Tet1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

    Plasmid

    #138417

    Purpose

    CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Inserts

    BirA
    dCas9
    

    Use

    Lentiviral

    Tags

    FLAG, Avi-tag and V5, His

    Promoter

    EF1a

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. cytoplasmic cpVenus control

    Plasmid

    #186788

    Purpose

    Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-HA C/EBP gamma

    Plasmid

    #15715

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    C/EBP gamma (CEBPG Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only