159,557 results

1. pHBS1147 GFP-TDP43

   Plasmid

   #118797

   Purpose

   To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

   Plasmid

   #105648

   Purpose

   Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

   Depositor

   Sandra Rieger

   Insert

   Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP SNAP23

   Plasmid

   #101914

   Purpose

   Expresses SNAP23 in mammalian cells

   Depositor

   Thierry Galli

   Article

   Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

   Insert

   SNAP 23 (SNAP23 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Myc-NLRP3(R260W)

   Plasmid

   #73956

   Purpose

   Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

   Insert

   NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   R260W

   Promoter

   CMV iE

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAV-CAG-oScarlet-WPRE-BC1-pA

   Plasmid

   #220765

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al bioRxiv 2024.04.24.590975

   Insert

   oScarlert-WPRE-BC1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV-CAG-oScarlet-WPRE-BC2-pA

   Plasmid

   #220766

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al bioRxiv 2024.04.24.590975

   Insert

   oScarlert-WPRE-BC2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AiP13098 - pAAV-AiE0380h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN3098)

   Plasmid

   #208123

   Purpose

   Direct-expressing SYFP2 in astrocytes AAV Virus

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Mich et al bioRxiv 2023.09.20.558718

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSCALPS-FLAG-HA-mmuMettl3 APPA

   Plasmid

   #207124

   Purpose

   Lentiviral plasmid to express mouse Mettl3 APPA mutant

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   Mettl3 (Mettl3 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mutation

   D395A and W398A

   Promoter

   SSFV

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NES-EGFP-PH-ARNO2G-I303Ex2

   Plasmid

   #116868

   Purpose

   PIP3 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   CYTH2 (CYTH2 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amind acids 252-399, isoleucine 303 changed to gl…

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-C1(2)delta

    Plasmid

    #21216

    Depositor

    Tobias Meyer

    Article

    Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

    Insert

    GFP-N2-PKCdelta-C1 (Prkcd Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C1(2) domains of PKC delta (rat) (Lab plasmid WC…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-hCDC6p-Venus-puro

    Plasmid

    #212666

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLSL.R.Ly

    Plasmid

    #227697

    Purpose

    Reengineered geminiviral replicon vector

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNLS-iRFP670

    Plasmid

    #45466

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmirGLO-3'UTR-TNF

    Plasmid

    #153069

    Purpose

    Human TNF 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of TNF gene (TNF Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223_KRAS_p.G12S

    Plasmid

    #81654

    Purpose

    Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G12S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge