We narrowed to 5,494 results for: SPI

1. pJS278

   Plasmid

   #134139

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 132-255, R155A

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

   Plasmid

   #175018

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   OK-HEF s57a ED
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   s57a esterase knock out mutant

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST15 hSlp4-a

   Plasmid

   #40046

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp4-a (SYTL4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-dLbCpf1-BE0ΔiNLS

   Plasmid

   #107684

   Purpose

   Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0ΔiNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D832A/E925A/D1148A

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDK480

   Plasmid

   #134197

   Purpose

   Mammalian expression of Flip in SKAP and IRES GFP

   Depositor

   Iain Cheeseman

   Article

   Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

   Insert

   untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Alternate Start, G5 siRNA hardened

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRVH1-hygro shStx3

   Plasmid

   #40071

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Stx3 shRNA-1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCRII-Topo Diap3 in situ probe

   Plasmid

   #45602

   Depositor

   Jeffrey Macklis

   Article

   Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

   Insert

   Diap3 in situ probe (Diaph3 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 1589-2321 of BC028920

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCRII-Topo Crim1 in situ probe

   Plasmid

   #45600

   Depositor

   Jeffrey Macklis

   Article

   Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

   Insert

   Crim1 in situ probe (Crim1 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   insert contains bp#4062-4502 of NM_015800.3

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pRSF-G1(7CNW)RS

   Plasmid

   #222874

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tom20-ECFP-FRB

    Plasmid

    #210289

    Purpose

    mitochondrial OM marker and "anchor" in FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-ECFP-FRB
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ECFP-FRB-Sec61b

    Plasmid

    #210290

    Purpose

    nucleus OM marker and "anchor" for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    ECFP-FRB-Sec61b
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tom20-EYFP-miLID

    Plasmid

    #210291

    Purpose

    mitochondria marker and "anchor" for optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-EYFP-miLID
    

    Use

    Tags

    EYFP and iLID

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mActA-mSspB-mCherry

    Plasmid

    #210294

    Purpose

    ActuAtor for iLID optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    mActA-,SspB-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-G1(5CNW)RS

    Plasmid

    #222872

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEMS2075

    Plasmid

    #158098

    Purpose

    High Copy number plasmid containing smCBA insert.

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    smCBA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

    Plasmid

    #194230

    Purpose

    Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB3-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E45D mutation in LCB3

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

    Plasmid

    #194232

    Purpose

    Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-LCB3-PDGFR TMD-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L2111-FKBP-GPA-20-11S

    Plasmid

    #222882

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L2375-FKBP-GPA-20-11S

    Plasmid

    #222897

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L2376-FKBP-GPA-20-114

    Plasmid

    #222896

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge