4,471 results
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pX330a dCas9-LSD1
Plasmid#92362PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.DepositorInsertdCas9-LSD1
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRC-APEX2
Plasmid#72558PurposeExpression of cytosolic APEX2 in bacteriaDepositorInsertAPEX2
TagsHis6ExpressionBacterialMutationA134P relative to APEXAvailable SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
ExpressionMammalianAvailable SinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-lux
Plasmid#64963Purposemini-Tn7 luxCDABE transcriptional fusion vectorDepositorInsertlux operon
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
DRD2-Tango
Plasmid#66269PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI-D10R-E48R
TagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1219
Plasmid#61250PurposeCRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegansDepositorInsertsgRNA(F+E)
UseCRISPRExpressionWormPromoterU6 promoterAvailable SinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-Flpo
Plasmid#60663PurposeExpresses pAAV-hSynapsin-FlpoDepositorInsertFlpo
UseAAVPromoterhSynapsin1Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB-wt
Plasmid#58905Purposemammalian expression of wild type human RbDepositorAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP
Plasmid#48078PurposeMammalian expression of GFP tagged MeCP2-WTDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
TagsGFPExpressionMammalianMutationwild typePromoterCMVAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA1-CH-mneo
Plasmid#51288Purposecontains the fully codon optimized ORF1 and ORF2 of the human L1RPDepositorInsertL1 (L1RE1 Human)
TagsmneoI indicator cassette containing an inverted n…ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
TagsHAAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0 FLAG TRPV4
Plasmid#45751DepositorAvailable SinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP3
Plasmid#42310DepositorAvailable SinceFeb. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-ArchT-tdTomato
Plasmid#28305PurposeCre-dependent viral expression of ArchT-tdTomato for neuronal inhibitionDepositorHas ServiceAAV5InsertArchT-tdTomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdTomatoExpressionMammalianMutationN/APromoterCAGAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only
