20,093 results

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Flag-hPIASx alpha

Plasmid

#15209

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx alpha (PIAS2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCAFNF-GFP

Plasmid

#13772

Purpose

Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFP

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pLV.ATMi

Plasmid

#14542

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATM shRNA (ATM Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR2-CFP

Plasmid

#13015

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 2 (TLR2 Human)

Tags

CFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 S727A

Plasmid

#12304

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727A (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727A

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-E2F4

Plasmid

#10914

Depositor

Bob Weinberg

Article

Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

Insert

E2F4 (E2F4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only
pYESir2-puro

Plasmid

#1769

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Article

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDAC439

Plasmid

#195237

Purpose

Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190033

Purpose

vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13X

Expression

Mammalian

Mutation

Y672A,Y676A

Promoter

CBh, SV40, hU6

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.27_ARE/NRF2-SPE

Plasmid

#177775

Purpose

Luciferase reporter for ARE/NRF2

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

NRF2 (NFE2L2 Human)

Use

Luciferase

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-PIP-FUCCI

Plasmid

#138714

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMal-T-Avi-His/BirA

Plasmid

#102962

Purpose

Vector for in vivo biotinylation of a fusion protein in bacteria.

Depositor

Tonia Rex

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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HaloTag-bActinCDS-bActinUTR-MS2V5

Plasmid

#102718

Purpose

Halo-Actin reporter for detection of both mRNA and protein

Depositor

Robert Singer

Article

Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

HaloTag-bActinCDS-bActin3'UTR-MS2V5

Use

Lentiviral

Tags

HaloTag and MS2V5

Promoter

UbC

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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