161,123 results

1. CMV BARK p2A mCherry

   Plasmid

   #117689

   Purpose

   CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs peptide
   

   Use

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-HA-ER WT

   Plasmid

   #49498

   Purpose

   Expresses HA-tagged ER wild-type in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPSup

   Plasmid

   #149416

   Purpose

   Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNLS-iRFP670

   Plasmid

   #45466

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

   Plasmid

   #125256

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

   Depositor

   Roger D. Kamm

   Article

   Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

   Insert

   hChR2(H134R)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-forward

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSYC-187

   Plasmid

   #74794

   Purpose

   pCS4+-P2A-mCherry-CAAX

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV BARK D110A p2A mCherry

   Plasmid

   #117690

   Purpose

   CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVpuroATT

   Plasmid

   #47539

   Purpose

   Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

   Depositor

   David Weinstock

   Article

   Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

10. T7-VEE-mCherry

    Plasmid

    #193355

    Purpose

    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiDP1.2

    Plasmid

    #171095

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, Geminin, miniAID, mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ub-G76V-GFP

    Plasmid

    #11941

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-G76V-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC3A2

    Plasmid

    #132213

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC3A2 (SLC3A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pVSV eGFP RABV-G

    Plasmid

    #31833

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF3194

    Plasmid

    #144670

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TLX3 (TLX3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCX459

    Plasmid

    #229214

    Purpose

    CRISPR array plasmid for MAP1B (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for MAP1B (Spacers 1-24)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sec61b-V5-TurboID

    Plasmid

    #166971

    Purpose

    Expresses ER targeted TurboID for secretome biotinylation

    Depositor

    Hyun-Woo Rhee

    Article

    Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

    Insert

    Sec61b-V5-TurboID
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-HA-mSTING (V154M)

    Plasmid

    #214159

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V154M) (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

    Plasmid

    #85428

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-12
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-NES-PhoCl2f-mCherry

    Plasmid

    #164048

    Purpose

    Optogenetic control of protein localization by PhoCl2f

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2f-mCherry
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Use

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUNIV-SIG-5HTR4D100A

    Plasmid

    #16312

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Aspartic Acid 100 changed to Alan…

    Promoter

    Available since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pEGFP-Kif2c

    Plasmid

    #208651

    Purpose

    Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (KIF2C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-GFPLaminA-L530P

    Plasmid

    #32710

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L530P

    Promoter

    CMV

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

26. spisPink chromoprotein

    Plasmid

    #117839

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, spisPink
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pST50Trc3-HISNDHFR

    Plasmid

    #63976

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis; TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRK5-HA-YFP-Raptor K840R

    Plasmid

    #204418

    Purpose

    Acetylation mutant for human raptor

    Depositor

    David Rubinsztein

    Article

    Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

    Insert

    Regulatory Associated Protein Of MTOR, Complex 1
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Lysine 840 to Arginine (K840R)

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRK5-HA-YFP-Raptor K1097R

    Plasmid

    #204417

    Purpose

    Acetylation mutant for human raptor

    Depositor

    David Rubinsztein

    Article

    Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

    Insert

    Regulatory Associated Protein Of MTOR, Complex 1
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Lysine 1097 to Arginine (K1097R)

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge