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pCAGGS-flpE-puro
Plasmid#20733PurposeMammalian expression of FLPe recombinase from the highly-expressed CAGGS promoterDepositorInsertFLPe recombinase
UseFlp/frtTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mEosEM
Plasmid#132708Purposeapplication in Correlative light-electron microscopyDepositorInsertmEosEM
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOZ-N-FH RAP80
Plasmid#27501DepositorInsertRAP80 (UIMC1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMOS005: superecliptic pHluorin (cytosolic)
Plasmid#163049Purposesuperecliptic pHluorin (cytosolic)DepositorInsertsuperecliptic pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCH49 (crRNA expression vector)
Plasmid#217338PurposeCROP-seq crRNA expression vector with GFP and neo markerDepositorInsertAsDR-filler-AsDR8, GFP-neo
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV_U6-ωRNA*_ISDra2-TnpB-mNG
Plasmid#212968PurposeAAV vector for encoding an ISDra2-TnpB driven by EFs promoterDepositorInsertsISDra2-TnpB-T2A-mNG,
ωRNA*
UseAAV and CRISPRTagsNLS and NLS-FLAGExpressionMammalianMutationPromoterEF1a, T7 and U6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37090PurposeExpresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-Mart1-SABR
Plasmid#119052Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBIDepositorInsertA0201-Mart1-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B mCherry
Plasmid#108857PurposeEncodes for the fluorescent protein mCherryDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 1
Plasmid#42540DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorInsertp16-INK4A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE Tet ONAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK232 (CMV-OsTIR1-PURO)
Plasmid#72834PurposeExpresses OsTIR1 under the control of the CMV promoterDepositorInsertAAVS1-CMV-OsTIR1-PURO-AAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1108
Plasmid#143254PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREBBP (CREBBP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
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CMV-OMMjRCaMP-P2A-mito4xGCaMP6f
Plasmid#127872PurposeDual expression vector for mitochondrial matrix and outer membrane Ca2+ sensorsDepositorInsertOuter membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL-MHCK7-GCaMP6
Plasmid#65042PurposeLentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7DepositorInsertGCaMP6
UseLentiviralTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianMutationPromoterMHCK7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
UseTagsStrepII, TEVExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV
Plasmid#24828DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHEE2E-TRI
Plasmid#71288PurposeEgg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPCDepositorInsertssgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJYS2_crtYf
Plasmid#85544PurposeConstitutive transcription of crRNA of crtYf, Spectinomycin resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0725
Plasmid#143071PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKB2 (NFKB2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only
