We narrowed to 11,210 results for: nar

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p3XFLAG-CMV 7.1_syn6

Plasmid

#50012

Purpose

Encodes syntaxin 6 with a N-terminal 3X FLAG tag

Depositor

David Hackstadt

Article

Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

Insert

syntaxin 6 (Stx6 Rat)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBTK1024 (pDS-Def1)

Plasmid

#183125

Purpose

Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

Fragment of Def1 gene

Use

Synthetic Biology

Available Since

May 19, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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DmTmem63

Plasmid

#136598

Purpose

Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

DmTMEM63

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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XW09

Plasmid

#65833

Purpose

Punc-4c-QS-SL2-mcherry3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-Vinculin

Plasmid

#80024

Purpose

Mammalian expression of mCherry-vinculin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
phREX1-Luc

Plasmid

#17222

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human REX1 (ZFP42 Human)

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pINTO-N-SA

Plasmid

#61723

Purpose

Inducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Type

Empty backbone

Tags

mSA

Expression

Mammalian

Promoter

T-REx (CMV + 2xTetO2)

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBAV150

Plasmid

#79764

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Jean Greenberg

Article

Vinatzer et al Mol Microbiol. 2006 Oct;62(1):26-44. Epu

Type

Empty backbone

Use

Tetr

Expression

Plant

Promoter

DEX

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSL-1

Plasmid

#180422

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Red Chromprotein gene

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-HIS-ybbr

Plasmid

#101238

Purpose

SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

SdrG_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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PHBA02

Plasmid

#83536

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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XW08

Plasmid

#65832

Purpose

Punc-4-QF-SL2-mcherry-3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Tags

mCherry

Expression

Worm

Promoter

Punc-4

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA1

Plasmid

#199727

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA1 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmNFM

Plasmid

#83126

Purpose

Mouse neurofilament protein M cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFM (Nefm Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-LacZshRNA

Plasmid

#73977

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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XW52

Plasmid

#65838

Purpose

XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Czipper-QF-AD-NLS

Tags

mCherry

Expression

Worm

Promoter

Pmig-13

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pINTO-N-FH

Plasmid

#65924

Purpose

Inducible expression of proteins fused to an N-terminal Flag-HA tag (FH)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Type

Empty backbone

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV/TetO2 (T-REx)

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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