We narrowed to 17,157 results for: REV

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Flrt3-Fc-His

Plasmid

#72075

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt3 (Flrt3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Smo-YFP-DEST

Plasmid

#49099

Purpose

Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Smo (Smo Mouse)

Tags

YFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pSems LifeAct-StayGold

Plasmid

#222947

Purpose

Expression of LifeAct tagged with StayGold in mammalian cells

Depositor

Jacob Piehler

Article

Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

Insert

LifeAct

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFlp-Gm

Plasmid

#222335

Purpose

Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAG424-10X-tRNA

Plasmid

#70125

Purpose

Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Expression

Yeast

Promoter

None – SUP4 pol III promoter provided by insert

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pKH-MoHyPer

Plasmid

#78267

Purpose

Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

Depositor

Jeffrey Caplan, Nicole Donofrio

Article

Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

Insert

MoHyper

Use

Express in magnaporthe

Expression

Yeast

Promoter

Rp27

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only
pGC180

Plasmid

#19616

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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p406GALL

Plasmid

#17434

Depositor

Nicolas Buchler, Fred Cross

Insert

GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GALL promoter is between SacI and XbaI restrictio…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pYTK027

Plasmid

#65134

Purpose

Encodes pREV1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pREV1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pGC320

Plasmid

#19639

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro

Plasmid

#254456

Purpose

TK4 PiggyBac plasmid for stable expression of Zim3-dCas9 during human pluripotent stem cell differentiation

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

Zim3-dCas9-2xMyc-NLS-T2A-mApple
hygroR

Use

Piggybac

Tags

T2A-mApple

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

Plasmid

#201681

Purpose

Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

Depositor

Emma Rawlins

Article

Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

Inserts

SFTPC promoter region (2kb upstream)
TagRFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

Plasmid

#196191

Purpose

Tagging human SOX2 gene with P2A-EGFP at the C-terminus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)

Use

Homology repair donor

Mutation

G2913A mutation to replace the guide cutting site…

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Cytosolic Fyn FRET Biosensor

Plasmid

#124654

Purpose

FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NES

Plasmid

#175008

Purpose

Cytosol-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Sema7a-Fc-His

Plasmid

#72175

Purpose

Expresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema7a (Sema7a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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