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pCDH-TfR-mApple

Plasmid

#179384

Purpose

Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

Depositor

Angus Johnston

Publication

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

Transferrin receptor 1 - mApple (TFRC Human, Synthetic)

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-Puro-TDP-43-Q331K

Plasmid

#133761

Purpose

Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed glutamine 331 to lysine.

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Mutation

intracellular domain *

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLX304-YAP1 _TA

Plasmid

#59143

Purpose

YAP1 mutants that harbor a deletion of the transcriptional activation domain

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

TA (transactivation) domain deleted; aa291-497

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Active Wnt11-V5

Plasmid

#43824

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human WNT11 (WNT11 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1 Flag mHMGB2

Plasmid

#31610

Depositor

Yasuhiko Kawakami

Publication

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB2 (Hmgb2 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
APOBEC3G_pLX307

Plasmid

#98345

Purpose

Lentiviral expression of APOBEC3G

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

APOBEC3G (APOBEC3G Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-FGR

Plasmid

#23877

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGR (FGR Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Publication

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C9orf72 (C9orf72 Human)

Tags

GFP

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC78

Plasmid

#62339

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFRT-TODestFLAGHAhTTP

Plasmid

#48738

Purpose

Expresses hTTP

Depositor

Thomas Tuschl

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TTP (ZFP36 Human)

Tags

FLAG, HA, and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-N-Flag-HA-GFP

Plasmid

#41034

Purpose

Retroviral expression of GFP in mammalian cells

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Gateway Cloning and Retroviral

Tags

Flag, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
h-beta-ENaC

Plasmid

#83429

Purpose

full length human beta ENaC with V5 tag

Depositor

Christie Thomas

Publication

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human beta ENaC (SCNN1B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p5E-acta2

Plasmid

#78686

Purpose

acta2 promoter for Gateway cloning

Depositor

Sarah Childs

Publication

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTRANS_221

Plasmid

#91115

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Publication

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Publication

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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