We narrowed to 6,627 results for: hwa

1. pSecTaq2-hProS-Myc-6xHis

   Plasmid

   #226524

   Purpose

   Expresses TAM Receptor Ligand human PROS1

   Depositor

   Sergei Kotenko, Raymond Birge

   Article

   Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

   Insert

   Human PROS1 (PROS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

   Plasmid

   #223965

   Purpose

   Dual fluorescent reporter for histone H2B and for Cdt1

   Depositor

   Hee Won Yang

   Article

   Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

   Insert

   H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1-hVAP-B KD/MD

   Plasmid

   #104450

   Purpose

   Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   changed K87 to D and M89 to D

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Notch3 intracellular domain-pcw107-V5

   Plasmid

   #64623

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   NOTCH3 (NOTCH3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   intracellular domain *

   Promoter

   PGK

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5-FRT/T0-Flag-53BP1

   Plasmid

   #52507

   Purpose

   mammalian expression vector of 53BP1

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V128A and siRNA resistant sequence: ACGCGCTGACGAC…

   Promoter

   CMV/TO

   Available Since

   March 26, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCS2 LRP6-APEX2-Ires-eGFP-PAC

   Plasmid

   #180142

   Purpose

   used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

   Depositor

   Edward De Robertis

   Article

   Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

   Insert

   Human LRP6 (LRP6 Human)
   

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. eGFP-proα2(I)-G610C

   Plasmid

   #119827

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

   Plasmid

   #196185

   Purpose

   Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-EFa1-HA-Jag1

   Plasmid

   #46051

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Jag1 (Jag1 Rat)
   

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-hs-KRAS

    Plasmid

    #52729

    Purpose

    retroviral expression of human KRAS

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. G protein alpha-s(alpha3beta5)

    Plasmid

    #55799

    Purpose

    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…

    Promoter

    CMV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-hPKL WT

    Plasmid

    #107159

    Purpose

    For mammalian expression of Flag-hPKL WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    Flag-hPKL WT (PKLR Human)
    

    Use

    Retroviral

    Tags

    N-term Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NLuc-GID4

    Plasmid

    #200878

    Purpose

    Mammalian expression of human GID4 with N-terminal NanoLuciferase fusion

    Depositor

    Stefan Knapp

    Article

    Owens et al bioRxiv 2023.01.17.524225

    Insert

    NanoLuciferase-GID4 (C17orf39 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-3xFLAG-RSL1D1(WT)

    Plasmid

    #122301

    Purpose

    expresses RSL1D1 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. G protein alpha-s(alpha3beta5/G226A)

    Plasmid

    #55798

    Purpose

    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A in alpha…

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

    Plasmid

    #182425

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-hGH (GH1 Human)
    VNp-LZ
    

    Tags

    Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Caspase-8 (C360A)-pcw107-V5

    Plasmid

    #64632

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CASP8 (transcript variant B) (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    C360A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

18. U6-sgR26-eCas9-T2A-BlastR

    Plasmid

    #172493

    Purpose

    For CRISPR-assisted HDR, Rosa26 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-FLAG-IRES-blasticidin-Sox2 WT

    Plasmid

    #182046

    Purpose

    expression of Sox2

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107576

    Purpose

    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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