We narrowed to 11,742 results for: pcDNA

1. pcDNA5-FRT/TO-mNeonGreen-BRD4

   Plasmid

   #204692

   Purpose

   This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 deltaN47 Beta-catenin

   Plasmid

   #19287

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-47

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO GFP DNAJB6b

   Plasmid

   #19501

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJB6b (DNAJB6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-N-Myc_535-888 USP7

   Plasmid

   #131248

   Purpose

   Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-N-Myc_884-1102 USP7

   Plasmid

   #131249

   Purpose

   Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Venus/Cerulean-Synaptopodin-A in pcDNA6

   Plasmid

   #190798

   Purpose

   Imaging of synaptopodin in live cells

   Depositor

   Vivian Tang

   Article

   Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

   Insert

   Synaptopodin-A (SYNPO Human, Synthetic)
   

   Use

   Tags

   Cerulean, His, Venus, and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-TO-Puromycin-mVenus-MAP

   Plasmid

   #44118

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Use

   Tags

   mVenus-MAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Akt-STOPS-NLS

   Plasmid

   #175007

   Purpose

   Nuclear-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 Flag TSC2 (S939A)

   Plasmid

   #14132

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 S939A (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S939A.

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5/FRT/TO GFP DNAJB6a

    Plasmid

    #19502

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB6a (DNAJB6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3-V5-PTPN14-wild type

    Plasmid

    #61003

    Purpose

    Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA FLAG TORC1 (1-634)

    Plasmid

    #25718

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

    Insert

    TORC1 (1-634) (CRTC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-AU1-mTOR-E2419K

    Plasmid

    #19994

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-E2419K (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Glutamine 2419 to Lysine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1/opto-b2AR-EYFP

    Plasmid

    #20948

    Purpose

    Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Mar 18. ():.

    Insert

    opto-b2AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Use

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GFP-Flag-UTR

    Plasmid

    #183235

    Purpose

    To express FLAG tagged eGFP

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3puro-mGFP-FXR1a-sh5Resistant

    Plasmid

    #225448

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge