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pMXs-puro GFP-p62 D337, 338, 339A

Plasmid

#38280

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed D337A, D338A, D339A

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Article

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Human, Mouse)

Use

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62 dC

Plasmid

#38282

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Truncate mutant 1-265 amino acids residues

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT

Plasmid

#80951

Purpose

Expresses Gsdmd-NT in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT (Gsdmd Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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XE141 sFRP-1-CS2+

Plasmid

#16693

Depositor

Randall Moon

Insert

sFRP-1 (Sfrp1 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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HA-Foxo1 (pCMV5)

Plasmid

#12142

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 (Foxo1 Mouse)

Use

Tags

HA and Myc

Expression

Mammalian

Mutation

Wild-type

Promoter

CMV

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSKM

Plasmid

#136541

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCytERM-BDFP1.8-N1

Plasmid

#197186

Purpose

Expresses the fusion protein of CytERM-BDFP1.8 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-BDFP1.8-

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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p-Tet-O-Sox5-Puro

Plasmid

#221667

Purpose

Expresses Sox5 in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

Sox5 (Sox5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-BRD4

Plasmid

#183939

Purpose

Transcriptional co-activator BRD4 labeled by mCherry

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

mCherry-BRD4 (Brd4 Synthetic, Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

Plasmid

#194880

Purpose

Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Use

Tags

C-term v5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER

Plasmid

#222189

Purpose

Bacterial expression of mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMK287 (mAID-Hygro)

Plasmid

#72825

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Hygro

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-JunWT-Myc

Plasmid

#47443

Purpose

mammalian expression of WT c-Jun

Depositor

Axel Behrens

Article

Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

Insert

Jun (Jun Mouse)

Use

Tags

Flag and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-myc-CAR-2A-Thy1.1

Plasmid

#127890

Purpose

Expresses CART receptor along with tags.

Depositor

Anjana Rao

Article

Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

Inserts

myc,
CAR
2A
Thy1.1

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.GPI

Plasmid

#178335

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

Plasmid

#112837

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

SH2 + SH3 (C-term) domains of Grb2

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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