We narrowed to 2,682 results for: KL

1. Akaluc-N1

   Plasmid

   #120371

   Purpose

   Akaluc luciferase mammalian expression in -N1 backbone

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Akaluc (human optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human codon optimized

   Promoter

   CMV

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNAPII_CTD27-52

   Plasmid

   #98678

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACEBac1-PARP2-Flag-His-WT

   Plasmid

   #111574

   Purpose

   Expresses full length PARP2 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP2 (PARP2 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FU- dio PSD95-mCherry- W

   Plasmid

   #73919

   Purpose

   Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

   Depositor

   Elly Nedivi

   Article

   Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

   Insert

   PSD-95-mCherry
   

   Use

   Lentiviral

   Tags

   mCherry and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Ubi-C

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Actin Binding Domain Tension Sensor

   Plasmid

   #215368

   Purpose

   Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   Actin Binding Domain Tension Sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Actin Binding Domain Tensionless Control

   Plasmid

   #215371

   Purpose

   Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   Actin Binding Domain Tensionless Control
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH-EF1-GFP

   Plasmid

   #133018

   Purpose

   (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

   Depositor

   Michael Huen

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IPU-FLAG-NEK9

   Plasmid

   #168269

   Purpose

   Expresses NEK9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BRCA2 A12.2 gRNA

   Plasmid

   #90551

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A12.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FU- dio Teal-gephyrin - W

    Plasmid

    #73918

    Purpose

    To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    Teal-gephyrin
    

    Use

    Lentiviral

    Tags

    Teal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. VCP F12.4 gRNA

    Plasmid

    #90939

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F12.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMX-Gal4-TEAD3

    Plasmid

    #33106

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    TEAD3 (TEAD3 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. mTagRFP-T-H2B-6

    Plasmid

    #58019

    Purpose

    Localization: Nucleus/Histones, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pRN3P_EGFP_m6A_Tracer

    Plasmid

    #139403

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    m6A-Tracer
    

    Use

    In vitro transcription

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACEBac1-PARP1-Flag-His-WT

    Plasmid

    #111572

    Purpose

    Expresses full length PARP1 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mKate1.31-pBAD

    Plasmid

    #54827

    Purpose

    Localization: Protein Expression Vector , Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and mKate1.31

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid

    #192438

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-mCherry-Rheb(C181S)

    Plasmid

    #192437

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MASTL B4.4 gRNA

    Plasmid

    #90755

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MASTL (Guide Designation B4.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EZH2 E9.3 gRNA

    Plasmid

    #90684

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EZH2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    EZH2 (Guide Designation E9.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-mCherry-Rheb(5A)

    Plasmid

    #192439

    Purpose

    Encodes mCherry-tagged Rheb lacking residues 38-42.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb amino acids 38-42 deleted.

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CamKIIa:smFP-TVA-RGV

    Plasmid

    #202594

    Purpose

    Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

    Depositor

    Oleksandr Shcheglovitov

    Article

    Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

    Inserts

    smFP
    TVA
    RGV
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mEmerald-MCM4-C-19

    Plasmid

    #54167

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM4
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. E47 2/5-pCS2

    Plasmid

    #20914

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E47 2/5 (TCF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

26. mEmerald-MCM6-C-19

    Plasmid

    #54171

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM6
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

27. EZH2 E10.3 gRNA

    Plasmid

    #90685

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EZH2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    EZH2 (Guide Designation E10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. E12-pCLBabe

    Plasmid

    #20918

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E12 (TCF3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G155D

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mEmerald-MCM2-C-19

    Plasmid

    #54163

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM2
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge