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Showing: 601 - 630 of 22147 results

  3. pY036_ATP1A1_G5_Array

    Plasmid
    #86620
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86620-plasmid-map-sequence-id-168857

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  4. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86619-plasmid-map-sequence-id-168855

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  5. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187454
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187454-plasmid-map-sequence-id-367517

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  7. ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187457
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187457-plasmid-map-sequence-id-367465

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  10. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid
    #174721
    Purpose
    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    tdTomato
    aph
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and P2A linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174721-plasmid-map-sequence-id-349021

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  11. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  12. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid
    #187459
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187459-plasmid-map-sequence-id-367469

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  13. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Tags
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Promoter
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  15. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  16. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  18. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid
    #187456
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187456-plasmid-map-sequence-id-367464

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  19. INS-GFP-CD19-EPT

    Plasmid
    #210469
    Purpose
    Donor plasmid for knock-in GFP-CD19 into the human INS locus
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210469-plasmid-map-sequence-id-419052

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  20. ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

    Plasmid
    #187458
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187458-plasmid-map-sequence-id-367467

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  22. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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  24. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  25. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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Showing: 601 - 630 of 22147 results