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Showing: 1001 - 1050 of 9150 results

  1. pJOE6087.3

    Plasmid
    #135082
    Purpose
    pBR322-based L-rhamnose-inducible vector.
    Depositor
    Josef Altenbuchner
    Insert
    P-rhaP-BAD-eGFP-His tag
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135082-plasmid-map-sequence-id-266686

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  2. R4-LexAp65-R3

    Plasmid
    #41438
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    41438-plasmid-map-sequence-id-55248

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  3. pGLU_103

    Plasmid
    #225785
    Purpose
    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    Ptn5_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225785-plasmid-map-sequence-id-446997

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  4. pGLU_85

    Plasmid
    #225800
    Purpose
    This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    PmarC9_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225800-plasmid-map-sequence-id-446917

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  5. pKP996

    Plasmid
    #167654
    Purpose
    Express colicinB-His6, T18C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 T18C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with T18C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167654-plasmid-map-sequence-id-339834

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  6. pKP995

    Plasmid
    #167655
    Purpose
    Express colicinB-His6, M19C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 M19C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with M19C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167655-plasmid-map-sequence-id-339832

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  7. pKP997

    Plasmid
    #167656
    Purpose
    Express colicinB-His6, V20C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 V20C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with V20C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167656-plasmid-map-sequence-id-339829

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  8. pKP2264

    Plasmid
    #167660
    Purpose
    Express colicinB-His6, A121C, Y254C
    Depositor
    Kathleen Postle
    Insert
    cba-his6, A121C, Y254C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6, I121C, Y254C
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167660-plasmid-map-sequence-id-337305

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  9. pBT1-proD-mCherry

    Plasmid
    #65823
    Purpose
    Const. mCherry expression , proD promoter, E. coli Expression
    Depositor
    Michael Lynch
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    proD E . coli Constitutive Promoter
    Available since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65823-plasmid-map-sequence-id-111984

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  10. pZH_tdPP7cp_mCherry

    Plasmid
    #118267
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118267-plasmid-map-sequence-id-229509

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  11. pCMV-TALEN-Rep

    Plasmid
    #45964
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    45964-plasmid-map-sequence-id-64559

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  12. pZH_mPP7cp_mCerulean3

    Plasmid
    #118262
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCerulean3
    Depositor
    Zach Hensel
    Insert
    mPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118262-plasmid-map-sequence-id-229514

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  13. pZH_tdPP7cp_mCerulean3

    Plasmid
    #118263
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCerulean3
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118263-plasmid-map-sequence-id-229501

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  14. pZH_mPP7cp_mCherry

    Plasmid
    #118266
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    mPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118266-plasmid-map-sequence-id-229515

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  15. pZH_tdPP7cp_ECFP

    Plasmid
    #118265
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-ECFP
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118265-plasmid-map-sequence-id-229511

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  16. pZH_mPP7cp_ECFP

    Plasmid
    #118264
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-ECFP
    Depositor
    Zach Hensel
    Insert
    mPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118264-plasmid-map-sequence-id-229507

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  17. pCMV-Rab-Rep

    Plasmid
    #45967
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45967-plasmid-map-sequence-id-64561

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  19. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  20. pJBEI-6410

    Plasmid
    #47049
    Purpose
    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47049-plasmid-map-sequence-id-408250

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  21. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47048-plasmid-map-sequence-id-67070

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  22. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Chris Barnes
    Article
    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    133307-plasmid-map-sequence-id-272413

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  23. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  24. pRSF-Ivy-his-hLYZ

    Plasmid
    #83920
    Purpose
    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.
    Depositor
    Karl Griswold
    Article
    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do
    Inserts
    Ivy
    lysozyme (LYZ Human)
    Use
    Tags
    Ivy protein has C-terminal hisx6 tag
    Expression
    Bacterial
    Mutation
    Ivy leader sequence deleted and replaced.
    Promoter
    T7 and t7
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83920-plasmid-map-sequence-id-160356

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  26. pSMART-ala10

    Plasmid
    #87135
    Purpose
    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Article
    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.
    Inserts
    NADPH Alanine Dehydrogenase (ald Synthetic)
    alanine exporter (alaE Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D196A, L197R
    Promoter
    waaHp from E. coli as part of operon with ald* an…
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87135-plasmid-map-sequence-id-174451

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  27. PatoG-OG241

    Plasmid
    #72839
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoG
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72839-plasmid-map-sequence-id-133603

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  29. pRAR064

    Plasmid
    #185031
    Purpose
    400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nucleotides of rpmE 5'UTR scrambled, Only fi…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185031-plasmid-map-sequence-id-373311

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  31. pRAR102

    Plasmid
    #185033
    Purpose
    400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    U54C mutation of rpmE 5'UTR, Only first 90 n…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185033-plasmid-map-sequence-id-366954

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  32. pRAR106

    Plasmid
    #185036
    Purpose
    400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G79U mutation of rpmE 5'UTR, Only first 90 n…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185036-plasmid-map-sequence-id-374745

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  33. pRAR116

    Plasmid
    #185040
    Purpose
    400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    U58C and U70C of rpmE of 5'UTR, Only first 9…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185040-plasmid-map-sequence-id-363333

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  35. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Use
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

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  39. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Todd Klaenhammer, Rodolphe Barrangou
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

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  40. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  44. pET28a-Ec.coaA

    Plasmid
    #50386
    Purpose
    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.
    Depositor
    Erick Strauss
    Article
    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8
    Insert
    PanK
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50386-plasmid-map-sequence-id-75331

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  48. pTJV1ScKm-rpoB

    Plasmid
    #166980
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    NeoR/KanR
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166980-plasmid-map-sequence-id-331333

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Showing: 1001 - 1050 of 9150 results