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Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT
Plasmid#111072PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.5 AB (361-545, Δ395-511)/pET28HMT
Plasmid#111267PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.5 AB (361-545, Δ395-511)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1
Plasmid#111460PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/I346A, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1
Plasmid#111461PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/I346D, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1
Plasmid#111462PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/S536A/L540A, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1
Plasmid#111497PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/S536D/L540D, C643APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-42)
Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-40)
Plasmid#71876PurposeExpresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1 kb prom. + Enhancer CD274
Plasmid#107005PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationN.B. There are two published SNPs in this region …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-WT
Plasmid#182741PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ultra-Exon1Q145-Myc-A
Plasmid#110487PurposeLentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationPartial long 3'UTR wild type huntingtin exon…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ultra-Exon1Q23-Myc-A
Plasmid#110486PurposeLentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationShort 3'UTR wild type huntingtin exon1 fragm…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
UseTagsExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A3 WT
Plasmid#195849PurposeExpresses wild-type Homo sapiens ATP13A3DepositorInsertATPase 13A3 (ATP13A3 Human)
UseLentiviralTagsExpressionMutationD498N, L956P, M850I, V855M, R858H, L675VPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q23-Myc-B
Plasmid#110488PurposeLentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationshort 3'UTR mutant huntingtin exon1 fragment…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APPSw;T719N
Plasmid#177094PurposeFor lentiviral transduction of APPsw; T719NDepositorInsertAPPsw;T719N (APP Human)
UseLentiviralTagsExpressionMutationT719N, K651N and M652L (please see depositor comm…PromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q145-Myc-B
Plasmid#110489PurposeLentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationpartial long 3'UTR wild type huntingtin exon…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MSH6
Plasmid#215123PurposeExpresses AcGFP-MSH6 in mammalian cells from a retroviral vector.DepositorInsertMSH6 (MSH6 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-APP751
Plasmid#193772PurposeEntry vector for human APP isoform having 751 amino acid residuesDepositorInsertAPP (APP Human)
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)
Plasmid#198035PurposeUsed for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)DepositorInsertSINTBAD (TBKBP1 Human)
UseTagsEGFP and GST-TEVExpressionInsectMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FBXL17
Plasmid#236188PurposeExpression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.DepositorInsertFLAG-FBXL17 (FBXL17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FLAG-RGS14
Plasmid#236567PurposeExpresses Flag-RGS14 in mammalian cellsDepositorInsertHomo sapiens regulator of G-protein signaling 14 (RGS14 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-BACH1
Plasmid#232269PurposeExpression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.DepositorInsertBACH1 (BACH1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-FBXO22
Plasmid#232271PurposeExpression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell linesDepositorInsertFBXO22 (FBXO22 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG-BACH2
Plasmid#232275PurposeExpression of FLAG-tagged BACH2 (H. sapiens) in human cell linesDepositorInsertBACH2 (BACH2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-NbON10-NanoLuc
Plasmid#198691PurposeExpresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell cultureDepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, IgK leader sequence, NanoLuc, and twin…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-hsRUNX
Plasmid#205639PurposereRNA guide targeting Homo sapiens RUNXDepositorInsertreRNA-hsRUNX (RUNX1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ERAP1-rs27895(C)
Plasmid#206142PurposeExpresses the rs27895-C allele of ERAP1, myc-tagged.DepositorInsertERAP1 (ERAP1 Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3312
Plasmid#144788PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK4 (ELK4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0204
Plasmid#141554PurposeLentiviral vector for overexpressing the ELK4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK4 (ELK4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-d4
Plasmid#182743PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform delta 4 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-d13
Plasmid#182745PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform delta 13 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT
Plasmid#111398PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346APromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT
Plasmid#111399PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346DPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT
Plasmid#111400PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540APromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT
Plasmid#111451PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540DPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-BSD
Plasmid#224122PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-BSD markerDepositorInsertPE7-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)
Plasmid#224123PurposeMammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)DepositorInsertPE7-mutant (Q20A_Y23A_Y24F_F35A)
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationQ20A_Y23A_Y24F_F35A in La(SSB)1-194PromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNoROS-CAT
Plasmid#170152PurposeMammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidationDepositorInsertsUseGenome integration via sleeping beauty transposon…TagsFLAG tag and Nucleolar Localization Sequence (NoL…ExpressionMammalianMutationPromoterRPBSA and TRE promoterAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1444
Plasmid#143809PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARID4B (ARID4B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE
Plasmid#166970PurposeCre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.DepositorInsertHomo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
UseAAV and Cre/LoxTagsFLAG, T2A, and mCherryExpressionMammalianMutationPromoterhsynAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-APP-mGFP
Plasmid#196696PurposeExpresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsHA and mGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-WT
Plasmid#171377PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationPromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX5_ETV6
Plasmid#205881PurposeExpress mEGFP-tagged fusion protein, PAX5_ETV6 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX5_ELN
Plasmid#205879PurposeExpress mEGFP-tagged fusion protein, PAX5_ELN from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only
