Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,462 results for: U6 promoter

Clear keywords
Showing: 401 - 420 of 1462 results

  1. pLenLox_U6 Nlgn2

    Plasmid
    #59358
    Purpose
    Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Mouse, Rat)
    eGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and mouse U6
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    59358-plasmid-map-sequence-id-132852

    Enlarge

  4. ATP1A1_G4_Q118R_Dual_pegRNA

    Plasmid
    #173199
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173199-plasmid-map-sequence-id-339518

    Enlarge

  6. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    U6 and synthetic Probasin ARRx2
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68357-plasmid-map-sequence-id-122833

    Enlarge

  11. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #173211
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173211-plasmid-map-sequence-id-339506

    Enlarge

  12. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid
    #173212
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173212-plasmid-map-sequence-id-339521

    Enlarge

  13. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

    Plasmid
    #173213
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173213-plasmid-map-sequence-id-339522

    Enlarge

  16. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

    Enlarge

  19. pCFD3-NS

    Plasmid
    #149545
    Purpose
    Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Promoter
    U6
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149545-plasmid-map-sequence-id-306357

    Enlarge

  20. pCFD5-NS

    Plasmid
    #149546
    Purpose
    Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Promoter
    U6
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149546-plasmid-map-sequence-id-306364

    Enlarge

Showing: 401 - 420 of 1462 results