We narrowed to 15,858 results for: cas9

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Publication

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Mutation

Rice codon optimized

Promoter

2x35S

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAS9-mCherry-Frame +2

Plasmid

#66941

Purpose

CRISPaint frame selector +2

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA frame +2

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pSpCas9(BB)-2A-Neo

Plasmid

#127762

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

Plasmid

#73796

Purpose

pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

Depositor

Ronald Davis

Publication

Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

Inserts

dCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1, pRPR1(TetO), and pTef1

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Publication

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRZ-CAS9-mCherry

Plasmid

#80974

Purpose

Cas9, mCherry expression

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Expression

Mammalian

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Publication

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Promoter

dSV40 Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Publication

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Publication

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFREE_Cm

Plasmid

#92052

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMCS-rybozyme-IRES-CAS9

Plasmid

#64668

Purpose

Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

Depositor

Wataru Fujii

Publication

Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CAS9
HDV ribozyme

Use

CRISPR

Tags

Flag, Internal ribosome entry site, and guide RNA…

Expression

Mammalian

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCas9-Venus

Plasmid

#70267

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Publication

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

Cas9
Venus Reporter

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS and P2A

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Publication

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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