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Showing: 4181 - 4200 of 77185 results
  1. pMM0652

    Plasmid
    #129013
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects
    Depositor
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pTiGc

    Plasmid
    #78314
    Purpose
    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
    Depositor
    Inserts
    TurboFP635
    GFP
    Use
    Synthetic Biology; Mycobacterial replicating vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hsp60 and hsp60(ribo)
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. ZJOM82

    Plasmid
    #133648
    Purpose
    Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.
    Depositor
    Insert
    p1k-NAB2-mCherry (NAB2 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. ZJOM64

    Plasmid
    #133655
    Purpose
    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.
    Depositor
    Insert
    p1k-COX4-DuDre (COX4 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. ZJOM75

    Plasmid
    #133649
    Purpose
    Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Insert
    p1k-ANP1-mCherry (ANP1 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Expression
    Mutation
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. ZJOM40

    Plasmid
    #133650
    Purpose
    Integration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.
    Depositor
    Insert
    p1k-SEC7-DuDre (SEC7 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. ZJOM68

    Plasmid
    #133657
    Purpose
    Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.
    Depositor
    Insert
    p1k-TGL3-mCherry (TGL3 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 4181 - 4200 of 77185 results