We narrowed to 29,111 results for: PLE;

1. pCMV-dCas12a-A3B

   Plasmid

   #193642

   Purpose

   Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3B, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-A3G

   Plasmid

   #193643

   Purpose

   Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Lyn-sACt-mCherry

   Plasmid

   #138216

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   Lyn-sACt-mCherry (Adcy10 Rat)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7432 Bicistronic_GFP_ires_puro

   Plasmid

   #64336

   Purpose

   This a retroviral expression plasmid expressing GFP along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Opto-GPR27

   Plasmid

   #106013

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR27 (GPR27 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAPTrap-GFP

   Plasmid

   #82506

   Purpose

   Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS2166

   Plasmid

   #158412

   Purpose

   High Copy number plasmid containing Ple331 (PAX6 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple331 (PAX6 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple331

   Available since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pJZC41

   Plasmid

   #62332

   Purpose

   sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA
   PCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Opto-GPR150

   Plasmid

   #106050

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR150 (GPR150 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBlastR-MCS-QF-MiniWhite

    Plasmid

    #165906

    Purpose

    Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p.UTA.2.0 miR-27a-3p 3 perfect copies.

    Plasmid

    #82439

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE hygro MEN1 L22R

    Plasmid

    #11021

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 L22R (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L22R

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid

    #140227

    Purpose

    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HRE (Hypoxia inducible)

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJZC77

    Plasmid

    #62338

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pG418R-MCS-QF-MiniWhite

    Plasmid

    #165902

    Purpose

    G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJZC101

    Plasmid

    #62335

    Purpose

    sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE hygro MEN1 T344R

    Plasmid

    #11019

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 T344R (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T344R

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pJZC73

    Plasmid

    #62341

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMART-ala10

    Plasmid

    #87135

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Inserts

    NADPH Alanine Dehydrogenase (ald Synthetic)
    alanine exporter (alaE Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    waaHp from E. coli as part of operon with ald* an…

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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