We narrowed to 17,780 results for: REV

1. pHM103

   Plasmid

   #225527

   Purpose

   Encodes constitutive GFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

   Plasmid

   #227086

   Purpose

   Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

   Plasmid

   #227084

   Purpose

   Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   STREPii-FLAG and SV40 NLS 1

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CUP1-∆ssCPY*-mScarletI

   Plasmid

   #221157

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CUP1-∆ssCPY*-mNeonGreen

   Plasmid

   #221156

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   tRNA-Gln

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HCP4

   Plasmid

   #166106

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNSU299_RPL39

   Plasmid

   #166078

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HCP6

   Plasmid

   #166108

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNSU299_YDR344C

    Plasmid

    #166072

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNSU299_TDH3

    Plasmid

    #166084

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of TDH3 (TDH3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. yCp50-PRP5

    Plasmid

    #111303

    Purpose

    Prp5 in yCp50 plasmid

    Depositor

    Christine Guthrie

    Article

    Noble et al Genetics. 1996 May;143(1):67-80.

    Insert

    PRP5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBSIIKS_HIS3_I

    Plasmid

    #109062

    Purpose

    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. sub2-5 pRS315

    Plasmid

    #111302

    Purpose

    sub2-5 in pRS315

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-5

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTK094

    Plasmid

    #65201

    Purpose

    Encodes HO 5' Homology as a Type 8b part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    HO 5' Homology
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTK088

    Plasmid

    #65195

    Purpose

    Encodes HO 3' Homology as a Type 7 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    HO 3' Homology
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYTK052

    Plasmid

    #65159

    Purpose

    Encodes tSSA1 as a Type 4 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    tSSA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYTK054

    Plasmid

    #65161

    Purpose

    Encodes tPGK1 as a Type 4 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    tPGK1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYTK055

    Plasmid

    #65162

    Purpose

    Encodes tENO2 as a Type 4 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    tENO2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYTK061

    Plasmid

    #65168

    Purpose

    Encodes tENO1 as a Type 4b part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    tENO1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge