We narrowed to 10,102 results for: otos

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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TRIN-shMYB.2652

Plasmid

#105575

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB shRNA #2652

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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TRIN-shMYB.2572

Plasmid

#105574

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB shRNA #2572

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pME-MLS-KillerRed

Plasmid

#115517

Purpose

Middle entry plasmid containing membrane bound KillerRed fluorophore

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Article

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCIpA102-G-HLA-A2_GFP

Plasmid

#85162

Purpose

for mRNA expression of HLA.A2-EGFP

Depositor

Tone Fredsvik Gregers, Sébastien Walchli

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Mrna expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209664

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eYFP

Plasmid

#104055

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

Insert

EYFP

Use

AAV

Promoter

CAG

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-25xUPRE-minP-EGFP

Plasmid

#159668

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xUPRE-minP-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-tdKatushka2-WPRE

Plasmid

#193344

Purpose

AAV-mediated expression of tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3V(1-10)

Plasmid

#117483

Purpose

Lentiviral vector for constitutive expression of sfCherry3V(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3V(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

6 substitutions (K45R, G52D, L83W, T106A, K182R, …

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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VPR-ps-dSpCas9

Plasmid

#102855

Purpose

A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

dSpCas9, pdDronpa1.2

Use

CRISPR and Synthetic Biology

Tags

NLS and VP64-p64-Rta transcription activator

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ac-AChBP

Plasmid

#63128

Purpose

Expression of Aplysia californica AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Ch-gelsolN

Plasmid

#37262

Depositor

Ikuo Wada

Article

Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

Insert

gelsolin (GSN Human)

Tags

mCherry

Expression

Mammalian

Mutation

deleted amino acids 380-731

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only