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Showing: 10551 - 10600 of 23135 results

  22. Prp2-pRSETA

    Plasmid
    #61042
    Purpose
    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
    Depositor
    Nils Walter
    Article
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Prp2
    Use
    Tags
    Histidine Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pET28-NFLtail

    Plasmid
    #232055
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFL tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Light tail domain
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232055-plasmid-map-sequence-id-464065

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  28. pET28-NFMshuf1

    Plasmid
    #232058
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 1
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232058-plasmid-map-sequence-id-464062

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  29. pET28-NFHtail

    Plasmid
    #232057
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232057-plasmid-map-sequence-id-464061

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  30. pET28-NFMshuf2

    Plasmid
    #232059
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 2
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232059-plasmid-map-sequence-id-464059

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  42. pGEM_Δflv3.sm

    Plasmid
    #185533
    Purpose
    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    aadA gene flanked by flv3 upstream and downstream regions
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185533-plasmid-map-sequence-id-371409

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  46. RT-4

    Plasmid
    #220443
    Purpose
    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.
    Depositor
    Marvin Wickens
    Article
    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do
    Insert
    PUF3 (PUF3 Budding Yeast)
    Use
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220443-plasmid-map-sequence-id-436941

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Showing: 10551 - 10600 of 23135 results