We narrowed to 9,777 results for: 158

1. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EYFP-JAB1

   Plasmid

   #111213

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Orel et al J Cell Mol Med. 2010 Jun;14(6B):1555-68.

   Insert

   CSN5 (COPS5 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-TetOne-Puro-LbNOX-FLAG

   Plasmid

   #218631

   Purpose

   This plasmid contains LbNOX under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. All-in-one lenti-evoCDA-NG

   Plasmid

   #200449

   Purpose

   Lentivirus vector for base editing

   Depositor

   Benjamin Cravatt

   Article

   Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   TaU3

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNP2270

   Plasmid

   #222733

   Purpose

   pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (lacZ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB_TRE3G_scFV-sfGFP-Prdm9CD

   Plasmid

   #235577

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Prdm9 (Prdm9 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. iCAP-ROOT

    Plasmid

    #192262

    Purpose

    AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

    Depositor

    Li Ye

    Article

    Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

    Insert

    Synthetic construct for ROOT VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

    Plasmid

    #193823

    Purpose

    CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    [4xCox8]-ChemoG-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LbCas12a-niCPE

    Plasmid

    #213747

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-nLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 gfaABC1D-Ezr-eGFP

    Plasmid

    #176856

    Purpose

    Expresses Ezrin fused with eGFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWzl_CRKL-Y207F+W275L

    Plasmid

    #196022

    Purpose

    Expresses CRKL Y207F + W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWzl_CRKL-W275L

    Plasmid

    #196019

    Purpose

    Expresses CRKL W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-A3-HA-ubiquitin

    Plasmid

    #220145

    Purpose

    Expresses A3-Ub for E3 substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    A3-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_SynP_DIO_tAKARα _WPRE

    Plasmid

    #196675

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    tAKARα
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. cpLID-micro

    Plasmid

    #168995

    Purpose

    circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    mCh-sspB-T2A-cpLID-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PYL1_nTEVp

    Plasmid

    #119213

    Purpose

    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nTEVp in sufion with PYL1
    

    Use

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABI_cTEVp

    Plasmid

    #119214

    Purpose

    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cTEVp in fusion with ABI
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCH61 (multiAsCas12a-KRAB piggyBac)

    Plasmid

    #217332

    Purpose

    piggyBac expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLIX403 RNaseH1

    Plasmid

    #237695

    Purpose

    Inducible lentiviral expression of RNaseH1

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    RNaseH1 (RNASEH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut

    Plasmid

    #235586

    Purpose

    Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    G282A

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pORTMAGE312B

    Plasmid

    #128969

    Purpose

    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    xylS-Pm

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid

    #117006

    Purpose

    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV_SynP_DIO_tAKARα- T391A_WPRE

    Plasmid

    #196676

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    tAKARα-T391A
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge