We narrowed to 9,629 results for: otos

1. Yellow Cameleon-Nano50/pcDNA3

   Plasmid

   #51964

   Purpose

   mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano50
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYFAC-CH3

   Plasmid

   #168979

   Purpose

   AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in asp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PBMN YFP-ER50

   Plasmid

   #37261

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al J Am Chem Soc. 2012 Mar 7;134(9):3942-5.

   Insert

   ER50 (ESR1 Human)
   

   Use

   Retroviral

   Tags

   HA-YFP

   Expression

   Mutation

   6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

   Promoter

   LTR

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-Chronos-mT-Sapphire-NRN

   Plasmid

   #149692

   Purpose

   For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

   Depositor

   Robert Malenka

   Article

   Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

   Insert

   Chronos
   

   Use

   AAV

   Tags

   mT-Sapphire

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-V5-mScarlet-I_SV40pA (JDW 968)

   Plasmid

   #224529

   Purpose

   A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-YPet-WPRE

   Plasmid

   #193342

   Purpose

   AAV-mediated expression of YPet under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   YPet
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC-SARS-CoV-2 NSP6

   Plasmid

   #158357

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP6 (ORF1ab )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGEX-KG-CAND1

   Plasmid

   #19949

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1(+)-mTagBFP2-MRTF-B

   Plasmid

   #182062

   Purpose

   Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mTagBFP2-MRTF-B (Mrtfb Mouse)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-Antares2

    Plasmid

    #120868

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-Antares2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Antares2 amplicon

    Promoter

    CAG Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209655

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Has Service

    AAV5

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

    Plasmid

    #168191

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-cofilin S3A

    Plasmid

    #163604

    Purpose

    Expresses constitutively active cofilin in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S3A substitution to make cofilin constitutively a…

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBR322-Pdp1_NTD-ZFD_R

    Plasmid

    #198277

    Purpose

    For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFD_R
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-FLAG-human NPM1-C1

    Plasmid

    #160693

    Purpose

    Mammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).

    Depositor

    Charles Sherr

    Article

    Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks NBD domain

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-mRuby3-WPRE

    Plasmid

    #193343

    Purpose

    AAV-mediated expression of mRuby3 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAFNF-ECFP-Actb

    Plasmid

    #169787

    Purpose

    An actin FRET sensor. Must be expressed with Venus-actin for FRET.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Actb (Actb Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC-SARS-CoV-2 N

    Plasmid

    #158370

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 N (N )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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