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LI A-B Ubi (+ intron) c4

Plasmid

#54408

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

L. japonicus polyubiquitin promoter

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem pA

Plasmid

#80823

Purpose

CMVmin-promoted GFPmem with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

3E-CMV-GFPCAAXpA spacer

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-NS

Plasmid

#80497

Purpose

Multisite gateway entry clone for adding N-terminal fusions of mVenus

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

mVenus (no STOP)

Use

Multisite gateway middle entry clone

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p70rg 1

Plasmid

#17828

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
mEGFP-30nmSAH-IQ

Plasmid

#61678

Purpose

GFP-labeled SAH scaffold with IQ attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-IQ

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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LI C-D destab GFP c1

Plasmid

#54454

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

destablized GFP

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-EF(N)-30nmSAH

Plasmid

#61679

Purpose

GFP-labeled SAH scaffold with EF Hand attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-EF(N)-30nmSAH

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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LI C-D Cerulean c1

Plasmid

#54417

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP Cerulean

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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p70rg 5

Plasmid

#17829

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pLIC.B3.TM1734

Plasmid

#11527

Depositor

Sung-Hou Kim

Article

Liu et al J Biol Chem. 2005 Apr 22. 280(16):15960-

Insert

PhoU (TM1734 T. maritima)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
SM420_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-TruSeq_3

Plasmid

#244224

Purpose

Vector for cloning LbCas12a crRNA array libraries. Contains 3' TruSeq sequence for easy NGS library construction

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mScarlet-I-QtSig

Plasmid

#236775

Purpose

Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

HA_DD-N_mScarlet-I_QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mTagBFP2-QtSig

Plasmid

#236776

Purpose

Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N, TagBFP, QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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SM425_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-KAN-DR

Plasmid

#244225

Purpose

Vector for golden gate cloning LbCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SM426_pHAGE-CAG-mCherry-T2A-puro-DR-As_crRNA-KAN-DR

Plasmid

#244226

Purpose

Vector for golden gate cloning AsCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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SM271_EF1A-dHyperLbCas12a-4xSID-T2A-BlastR

Plasmid

#244209

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

Plasmid

#244210

Purpose

Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

Plasmid

#244212

Purpose

Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastR

Plasmid

#244205

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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