169,724 results

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pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Tags

His and V5

Expression

Bacterial and Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX.TRE3G.AdProt

Plasmid

#131687

Purpose

A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus Protease

Use

Lentiviral

Promoter

TRE3GV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-RNF51

Plasmid

#126546

Purpose

Expresses GFP-tagged RNF51 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF51 (BMI1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE_puro

Plasmid

#118692

Purpose

Empty vector to express overexpression cDNA

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAV5S-F30-2xdBroccoli

Plasmid

#66845

Purpose

Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Tags

5S rRNA

Expression

Mammalian

Promoter

5S

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMW#3

Plasmid

#13350

Depositor

Marian Walhout

Article

Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

Insert

LacZ reporter

Expression

Yeast

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(T21N)

Plasmid

#99711

Purpose

Mammalian expression of HA-RagA(T21N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

HA

Mutation

T21N

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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cr3Vn

Plasmid

#184861

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, gam, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-sfGFP

Plasmid

#61058

Purpose

A direct fusion of sfGFP to the 24xGCN4_v4 peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v4 peptide array and sfGFP

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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single cut NH-donor

Plasmid

#83575

Purpose

eGFP reporter donor containing single SA cutting site for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Sec31a #861#640

Plasmid

#42123

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
K44A HA-dynamin 2 pcDNA3.1

Plasmid

#34685

Depositor

Sandra Schmid

Insert

dynamin 2ba

Tags

HA (variant)

Expression

Mammalian

Mutation

K44A

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV6 BDNF

Plasmid

#39857

Depositor

Yves-Alain Barde

Article

Hofer et al EMBO J. 1990 Aug;9(8):2459-64.

Insert

BDNF (Bdnf Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma1-GFP2

Plasmid

#140989

Purpose

Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma1-GFP2 (GNGT1 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ14901

Plasmid

#239276

Purpose

Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PspCas13b-hGAPDH-gRNA1 (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PLEXIN A1

Plasmid

#58237

Purpose

Riboprobe for in vitro transcription of Plexin A1

Depositor

Christiana Ruhrberg

Article

Bouvree et al Circ Res. 2012 Aug 3;111(4):437-45. doi:

Insert

Plexin A1 (Plxna1 Mouse)

Expression

Bacterial

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P1TDPGH023

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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mKikGR-C1

Plasmid

#54656

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Habuchi et al PLoS One. 2008;3(12):e3944. doi: 10.1371

Type

Empty backbone

Tags

mKikGR

Expression

Mammalian

Available Since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIP-CMV-GFP-FLAG-cGAS

Plasmid

#86675

Purpose

Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.8TM.BB.3z

Plasmid

#200671

Purpose

Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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dmBACCS2-IRES-dOrai

Plasmid

#67627

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Tags

6xHis (on sfGFP)

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
CBF:H2B-Venus

Plasmid

#44211

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

Insert

CBF1-Responsive-Element

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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p-miniTurbo-HA

Plasmid

#209637

Purpose

Expression miniTurbo-HA in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

miniTurbo

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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