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Plasmid#108510Purposeexpression of vsv-GCN4-dCEN-INCENPDepositorInsertGCN4-dCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationreplace CEN box for GCN4 dimerazation domainPromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg1
Plasmid#124856PurposeMutagenesis of Gabrg1DepositorInsertGabrg1 (Gabrg1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD
Plasmid#140304PurposeExpresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-KD
Plasmid#140296PurposeExpresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and KD regionsPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S32
Plasmid#140308PurposeExpresses HA-tagged human Src-S32 in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH3 and SH2 domainsPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin2b
Plasmid#124864PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-JUN
Plasmid#79512PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDSDepositorInsertJUN (JUN Human)
UseMultisite gateway entry cloneTagsHA TagExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-S18
Plasmid#127825PurposeProduction of recombinant human ribosomal protein S18DepositorInsertRPS18 (RPS18 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGex4t2 with 244-458AA SUUR fragment
Plasmid#112989PurposeExpresses fusion of GST and amino acids 244-458 of Drosophila SUURDepositorInsertSuUR (SuUR Fly)
UseTagsExpressionBacterialMutationamino acids 244-458PromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWp100
Plasmid#157977PurposepBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB1-1
Plasmid#121533PurposesgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB1-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB4-1
Plasmid#121535PurposesgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB4-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S32-KD
Plasmid#140310PurposeExpresses HA-tagged human Src-S32-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationContains only the SH3, SH2, and KD regionsPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB0323-TEV-His12
Plasmid#137039Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66700.1
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
EZH2-TALEN_Right_No_FLAG
Plasmid#79900PurposeUntagged EZH2-TALEN_Right to target the N-terminus of human EZH2DepositorInsertEZH2-TALEN_Right_No_FLAG (EZH2 Human)
UseTALENTagsExpressionMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAav-MCS-PQS1-3xFLAG
Plasmid#84883PurposerAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertMulticloning sites and selection cassette
UseAAVTagsExpressionMutationPromoterPGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH-SWAP70(R223E, R224E)
Plasmid#84583PurposeExpresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide bindingDepositorInsertSWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
UseTagsEGFPExpressionMammalianMutationPleckstrin-homology domain, residues 209-306, mut…PromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
vsv-CenpB 1-166-Sgo1 N61I-GFP
Plasmid#108499Purposeexpression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)DepositorInsertCenpB-Shugoshin 1 (SGO1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationCenpB 1-166 fusion before Sgo1, no PP2A binding N…PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E DN Kif5a
Plasmid#105973Purpose3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bpDepositorInsertKif5a (kif5aa Zebrafish)
UseTagsExpressionBacterialMutationtruncation of aa 416-1033PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W
Plasmid#105019PurposeLentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFPDepositorInsertVps39 (Vps39 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only