We narrowed to 10,893 results for: ENA;

1. pAAV-hSyn-ChRger3-TS-EYFP

   Plasmid

   #127240

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HaloTag-3xrGBD

   Plasmid

   #176108

   Purpose

   Visualization of active Rho

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   rhotekin (Rtkn Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GLG1-EGFP

   Plasmid

   #210372

   Purpose

   Expresses GLG1 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   GLG1 (GLG1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR_L1-YFP-miniTurbo-NES-L2

   Plasmid

   #127351

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINK-OTUD1 (full-length, aa 1-481)

   Plasmid

   #61405

   Purpose

   Expresses human OTUD1 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. psicheck2 PTEN 3'UTR

   Plasmid

   #50936

   Purpose

   reporter for PTEN RNAi

   Depositor

   Pier Pandolfi

   Article

   Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

   Insert

   PTEN 3'UTR (PTEN Human)
   

   Use

   RNAi

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

   Plasmid

   #164140

   Purpose

   AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

   Depositor

   Hirokazu Hirai, Hajime Hirase

   Article

   Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

   Insert

   R-CaMP1.07
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hGFAP

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-AAV-sh[SNCA]

   Plasmid

   #75437

   Purpose

   knockdown alpha-synuclein in rat neurons

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   snca (Snca Rat)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

   Plasmid

   #83925

   Purpose

   Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   eGFP
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Expression

   Mutation

   Promoter

   hUbC

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

    Plasmid

    #173873

    Purpose

    Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FgH1tUTG_mup53_Ex5

    Plasmid

    #85535

    Purpose

    Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-BirA-P2A-mCherry

    Plasmid

    #74056

    Purpose

    retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    BirA biotin ligase
    

    Use

    Retroviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMV_AA050

    Plasmid

    #216107

    Purpose

    gDNA expression for Cas9 with ChenF+E trRNA (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gDNA expression for Cas9 with ChenF+E trRNA
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_889

    Plasmid

    #216080

    Purpose

    ITRs from AAV2, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    ITRs from AAV2
    

    Use

    AAV; Destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-Cezanne (OTU, aa 53-446)

    Plasmid

    #61581

    Purpose

    Expresses human Cezanne (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne (OTUD7B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-52 and aa 447-843.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcU6_1 sgRNA

    Plasmid

    #92395

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.1 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chick U6.1

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-IKZF2-V2-V5

    Plasmid

    #107391

    Purpose

    Mammalian expression of IKZF2-V2-V5

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2-V2 (IKZF2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SauKKHCas9 PE2*

    Plasmid

    #169852

    Purpose

    Mammalian Expression, sauCas9-KKH based prime editor

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SaCas9KKH-N580A-M-MLV
    

    Use

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEY43

    Plasmid

    #191047

    Purpose

    eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    eat-4(prom6-1) (eat-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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