We narrowed to 6,547 results for: proc

1. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

   Plasmid

   #128698

   Purpose

   simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

   Inserts

   iqgC (full length)
   Raf1 kinase (aa 50-132)
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP and mRFP

   Expression

   Mutation

   none

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_hE2F7mg_6ntmut

   Plasmid

   #73075

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns wi…

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1_hE2F7mg_10ntmut

   Plasmid

   #73076

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns wi…

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Met1 diUb (1-152, R42W)

   Plasmid

   #110767

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   R42W

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCD1E

   Plasmid

   #102956

   Purpose

   gene cloning of human CD1E

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

   Insert

   CD1E cDNA (CD1E Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP1893-1

   Plasmid

   #105248

   Purpose

   human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdEos-EB3-7

   Plasmid

   #57610

   Purpose

   Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   EB3 (MAPRE3 Human)
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pEN_hU6miR-Gb2-K

   Plasmid

   #25759

   Purpose

   Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-puro-N-3HA-SREBP1-C-Flag

   Plasmid

   #134286

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220612

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet_huCAD_ICADL (TevS)

    Plasmid

    #100098

    Purpose

    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two caspase cleavage sites (DETD-117 and DAVD-224…

    Promoter

    T7

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV CD68-hM4D(Gi)-mCherry

    Plasmid

    #75033

    Purpose

    Expression of hM4D(Gi) using the CD68 promoter.

    Depositor

    Bryan Roth

    Article

    Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Has Service

    AAV1 and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CD68

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-puro-N-3HA-SREBP2-C-Flag

    Plasmid

    #134287

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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