We narrowed to 10,847 results for: ENA
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Plasmid#204605PurposeMammalian expression of adenine transversion base editor AXBEDepositorInsertbpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationS. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
Plasmid#173872PurposeMammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)DepositorInsertmCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-NES-L2
Plasmid#127349Purposegateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AA028
Plasmid#216008PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9 (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA029
Plasmid#216009PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9-NG (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHeACT-mEGFP-NLS
Plasmid#205003PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGFAP-Pink Flamindo-WPRE-SV40
Plasmid#178790PurposeAAV vector to express the red cAMP indicator in astrocytesDepositorInsertPink Flamindo
UseAAVTagsExpressionMutationPromoterhGFAPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
G1333 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157837Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST
Plasmid#127321PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)DepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W
Plasmid#105043PurposeLentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFPDepositorInsertStk11 (Stk11 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mito-LOV-Turbo-V5
Plasmid#199666Purposeexpresses LOV-Turbo in the mammalian mitochondria, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsCOX4I1 mito targeting sequence and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-ABEmax
Plasmid#164415PurposeEF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.DepositorInsertABEmax
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2101
Plasmid#91060PurposeModule B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRDB_181
Plasmid#216102PurposeCRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (full-length, aa 1-293)
Plasmid#61418PurposeExpresses human OTUD6B (full-length) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pII pm
Plasmid#218099PurposePhagemid that expresses LacI, and pII under the Lac promoterDepositorInsertpII
UseTagsExpressionBacterialMutationPromoterLac promoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only