We narrowed to 13,804 results for: TIM

1. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2xNLS-NLP-cMyc LbaCas12a

   Plasmid

   #182123

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc LbaCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB80-iLID-mCherry-RAB11

   Plasmid

   #174623

   Purpose

   Optogenetic coupling to recycling endosomes (RAB11) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   iLID-mCherry-RAB11 (RAB11A Human, Synthetic)
   

   Use

   Tags

   iLID-mCherry

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del; RAB11: Met1Del

   Promoter

   Chicken beta-actin

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MO70-hJunctate

   Plasmid

   #79596

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

   Insert

   junctate (ASPH Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSW002-PpsbA-mNeonGreen

   Plasmid

   #205017

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   PpsbA

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIM1 gRNA (BRDN0001146465)

   Plasmid

   #77174

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIM1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIM1 (PIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PIM1 gRNA (BRDN0001146597)

   Plasmid

   #77175

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIM1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIM1 (PIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PIM1 gRNA (BRDN0001145078)

   Plasmid

   #77176

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIM1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIM1 (PIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. WEE1 gRNA (BRDN0001149349)

   Plasmid

   #77367

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human WEE1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   WEE1 (WEE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. non-targeting control gRNA (BRDN0001147780)

    Plasmid

    #80226

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 7432 Bicistronic_GFP_ires_puro

    Plasmid

    #64336

    Purpose

    This a retroviral expression plasmid expressing GFP along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TT JunD-HA

    Plasmid

    #58498

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. WEE1 gRNA (BRDN0001145570)

    Plasmid

    #77368

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    WEE1 (WEE1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ROCK1 gRNA (BRDN0001147709)

    Plasmid

    #77593

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-HsSENP6

    Plasmid

    #71469

    Purpose

    Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 6 (SENP6 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA-hNgAgo

    Plasmid

    #104384

    Purpose

    human codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

    Insert

    hNgAgo
    

    Use

    Tags

    3XFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV10-Flag-Fbxl3

    Plasmid

    #48751

    Purpose

    Expression of FLAG-tagged Fbxl3

    Depositor

    Joseph Takahashi

    Article

    Siepka et al Cell. 2007 Jun 1;129(5):1011-23. Epub 20

    Insert

    Fbxl3 (Fbxl3 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

19. non-targeting control gRNA (BRDN0001145869)

    Plasmid

    #80256

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-G2ZYP2

    Plasmid

    #103110

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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