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pSP64-Lifeact-GFP-ePDZ

Plasmid

#135602

Purpose

Expression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Promoter

Unknown

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p5343_UC57

Plasmid

#126645

Purpose

environmental plasmid p5343 cloned into pUC57

Depositor

Aindrila Mukhopadhyay

Article

Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00

Insert

Environmental plasmid p5343

Use

Synthetic Biology

Promoter

NA

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PV246

Plasmid

#132337

Purpose

CasE constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

CasE

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV248

Plasmid

#132339

Purpose

CasD constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

CasD

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV384

Plasmid

#132344

Purpose

Cas9 DsRed targeting sgRNA expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 DsRed targeting sgRNA

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV422

Plasmid

#132345

Purpose

SthCascade DsRed targeting crRNA expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade DsRed targeting crRNA

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV521

Plasmid

#132346

Purpose

Cas9 R promoter targeting sgRNA1 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA1

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV523

Plasmid

#132347

Purpose

Cas9 R promoter targeting sgRNA2 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA2

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV528

Plasmid

#132348

Purpose

Cas9 R promoter targeting sgRNA3 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA3

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV564

Plasmid

#132349

Purpose

SthCascade R promoter targeting crRNA1 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade R promoter targeting crRNA1

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV565

Plasmid

#132350

Purpose

SthCascade R promoter targeting crRNA2 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade R promoter targeting crRNA2

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV569

Plasmid

#132351

Purpose

SthCascade R promoter targeting crRNA3 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade R promoter targeting crRNA3

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV244

Plasmid

#132335

Purpose

CasB constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

CasB

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV245

Plasmid

#132336

Purpose

CasC constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

CasC

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pCG6770 eGFP SV40

Plasmid

#132649

Purpose

Expresses eGFP mRNA from the Drosophila CG6770 promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

CG6770

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_ppst eGFP SV40

Plasmid

#132648

Purpose

Expresses eGFP mRNA from the Drosophila pst promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

pst

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pSirup eGFP SV40

Plasmid

#132647

Purpose

Expresses eGFP mRNA from the Drosophila Sirup promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Sirup

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pCG8620 eGFP SV40

Plasmid

#132646

Purpose

Expresses eGFP mRNA from the Drosophila CG8620 promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Hml

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pHml eGFP SV40

Plasmid

#132645

Purpose

Expresses eGFP mRNA from the Drosophila Hml promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Hml

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA eGFP MALAT1

Plasmid

#132643

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that ends in the MALAT1 triple helix

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSL1211

Plasmid

#129521

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFlag-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r76

Plasmid

#108084

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r76 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r76 (Vmn2r76 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-TAK1

Plasmid

#117162

Purpose

Target site AGCGCCCTTCAATGGAGGAAAT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-STAT2

Plasmid

#117161

Purpose

Target site TTTAAGTTCCACAGACTTGGA

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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ELK1 AB box pcDNA3.1/HisC

Plasmid

#121094

Purpose

Expression of the AB box fragment of ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

6xHis-omni-express

Expression

Mammalian

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9-J23113

Plasmid

#119701

Purpose

Expression of Cas9 with a weak promoter

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-U61-SapI

Plasmid

#112808

Purpose

gRNA cloning vector for expressing 1-3 gRNAs in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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LI F-G Tet

Plasmid

#104524

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Tetracycline resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI F-G Cml

Plasmid

#104525

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Chloramphenicol resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI Rsc202375 Upstream

Plasmid

#104526

Purpose

LI genomic upstream module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Rsc202375 genomic upstream region

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI Rsc203337 Downstream

Plasmid

#104527

Purpose

LI genomic downstream module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Rsc203337 genomic downstream region

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LIII 1-6 Amp RC

Plasmid

#104530

Purpose

LIII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHES945

Plasmid

#112643

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

CRY2PHR-GAL4DBD

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1100

Plasmid

#110117

Purpose

Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT dati MCS circfirefly_900

Plasmid

#110116

Purpose

Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT dati MCS circfirefly_700

Plasmid

#110115

Purpose

Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT dati MCS circfirefly_500

Plasmid

#110114

Purpose

Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mCFP

Plasmid

#105136

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mCFP

Tags

3nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn1r105

Plasmid

#105994

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Vmn1r105 (Gm5726, Vmn1r153, Gm8677 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mYFP

Plasmid

#105138

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mYFP

Tags

3nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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miR-722-Dendra pCDNA

Plasmid

#97163

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC102

Plasmid

#91176

Purpose

a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSC1

Plasmid

#91179

Purpose

T-DNA vector expressing TALENs targeting Medtr4g020620 and Medtr2g013650A

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALENs targeting Medtr4g020620 and Medtr2g013650A

Use

TALEN

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 10901 - 10950 of 25908 results

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We narrowed to 25,908 results for: Nes