We narrowed to 18,280 results for: Met;

1. pTag-RFP-C-h-Rab11a

   Plasmid

   #79806

   Purpose

   Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab11a (RAB11A Human)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

   Plasmid

   #218528

   Purpose

   sgRNA targeting human EIF2AK4/GCN2

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK4 (EIF2AK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC590 - pCMV ER-iRFP

   Plasmid

   #59136

   Purpose

   A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   ER-localized Infrared Fluorescent Protein
   

   Use

   Tags

   ER retention signal and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRB-TagRFPT-CAAX

   Plasmid

   #87710

   Purpose

   FRB-tagged TagRFPT; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FRB-TagRFPT-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA7-SacB

   Plasmid

   #79968

   Purpose

   TM-MAGE strain

   Depositor

   Morten Sommer

   Article

   Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

   Inserts

   Lambda Red recombinase beta subunit
   DNA adenine methylase
   Levansucrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pclbw-opa1(isoform 1)-myc

   Plasmid

   #62845

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform1-myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pMTS_mScarlet_N1

   Plasmid

   #85057

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   aa1-29

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-CRY2-CreN

   Plasmid

   #26888

   Purpose

   Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2-CreN (cre, CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 19-104 of Cre

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

   Plasmid

   #104571

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
   

   Use

   CRISPR

   Tags

   NLS-3xHA-3xFLAG-4xDmrA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid

    #182395

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & Leucine Zi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-STIM1-NN_neomycin

    Plasmid

    #65311

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Use

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS001: CMV-Optopatch2_FCK

    Plasmid

    #62984

    Purpose

    Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    Optopatch2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Tag4A-MMP8 Wt

    Plasmid

    #29545

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CaMKII-Chronos-GFP

    Plasmid

    #58805

    Purpose

    AAV expression of Chronos-GFP under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. mRuby2-MAPTau-C-10

    Plasmid

    #55904

    Purpose

    Localization: Microtubules, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MAPTau (MAPT Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Expression

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. DRH229: FCK-QuasAr1-mO2

    Plasmid

    #51629

    Purpose

    Fluorescent reporter for membrane voltage with improved brightness

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr1-mOrange
    

    Use

    Lentiviral

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

    Plasmid

    #35509

    Purpose

    AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV5 and AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn Con/Foff EYFP

    Plasmid

    #55651

    Purpose

    Cre-on/Flp-off EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp off eyfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV_NLS-SaCas9-NLS-VPR

    Plasmid

    #68496

    Purpose

    AAV vector containing SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SaCas9-VPR
    

    Use

    AAV and CRISPR

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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