We narrowed to 11,278 results for: VARS

1. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

   Plasmid

   #169333

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CABE-T3.155

   Plasmid

   #193270

   Purpose

   Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.155
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-Cre stuffer v4

   Plasmid

   #158033

   Purpose

   Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AY27_pU6.gRNA.CLYBL

   Plasmid

   #199238

   Purpose

   Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. pyogenes gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ230-RRV

   Plasmid

   #188543

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RRV
   

   Use

   CRISPR; Gateway compatible lbcas12a-rrv entry clo…

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, D156R, R182V

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2,0_3M5M_luciferase

   Plasmid

   #135475

   Purpose

   Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, luciferase reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-N-S202R

   Plasmid

   #177950

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S202R) (N )
   

   Expression

   Mammalian

   Mutation

   S202R

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC/CMV-TYK2-VSV

   Plasmid

   #139344

   Purpose

   Expression of TYK2 protein

   Depositor

   Sandra Pellegrini

   Article

   Li et al PLoS One. 2020 Jan 21;15(1):e0225289. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPT234

    Plasmid

    #127855

    Purpose

    Repressilator 2.0, without degradation tags, integrated triple reporter

    Depositor

    Johan Paulsson

    Article

    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

    Inserts

    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    First 11aa derived from mCherry as fusion protein

    Promoter

    pAmpR, pL LacO1, pL tetO1, and pR

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-ITGA6-1k-Basic-LUC

    Plasmid

    #107141

    Purpose

    Luciferase reporter plasmid to monitor transcriptional activity of canine ITGA6-promoter (1kb fragment)

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Insert

    ITGA6-promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CBE-T1.14

    Plasmid

    #193277

    Purpose

    Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.14
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)

    Plasmid

    #188379

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_NRG1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-P199L

    Plasmid

    #177949

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P199L) (N )
    

    Expression

    Mammalian

    Mutation

    P199L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-AID-BLM-UGI

    Plasmid

    #221218

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI
    

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LifeAct-14 (LA-14)

    Plasmid

    #158750

    Purpose

    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    LifeAct-14
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Deletion mutation for last three amino acids from…

    Promoter

    CMV immediate early

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GFP-FUS-IDR

    Plasmid

    #183236

    Purpose

    Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Tags

    FLAG tag and Fusion to an IDR from the FUS protei…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM4891 (pCV0003)

    Plasmid

    #120080

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Mutation

    mobA Y25F

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid

    #129594

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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