We narrowed to 27,263 results for: ANT

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pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

Plasmid

#107941

Purpose

Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a (E174R/S542R/K548R)

Tags

3x HA and NLS (nucleoplasmin)

Expression

Mammalian

Mutation

E174R, S542R and K548R

Promoter

CAG

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor Q63L (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor T19N (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Cdk5-DN-HA

Plasmid

#1873

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Tags

HA

Expression

Mammalian

Mutation

D145N, dominant negative

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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PKC delta DN

Plasmid

#16389

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta isoform 2 (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K376R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Article

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-rab9 DN

Plasmid

#12664

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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CoV2-M-IRES-E

Plasmid

#177938

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBB541

Plasmid

#27394

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

groESL (groL, groS E. coli)

Expression

Bacterial

Available Since

March 13, 2012

Availability

Academic Institutions and Nonprofits only

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pNW55

Plasmid

#22988

Purpose

Expression of miRNA precursor for the osa-MIR528 locus in rice.

Depositor

Detlef Weigel

Article

Warthmann et al PLoS One. 2008 . 3(3):e1829.

Insert

osa-MIR528

Use

RNAi; Plant gene inactivation

Expression

Plant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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