2,293 results

1. pHes1-DN-GFP (CC#110)

   Plasmid

   #15134

   Depositor

   Connie Cepko

   Article

   Furukawa et al Neuron. 2000 May . 26(2):383-94.

   Insert

   Hes1 (Hes1 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   E43A, K44A, R47A

   Available Since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

   Plasmid

   #104491

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP7s
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-Axon-jGCaMP8s

   Plasmid

   #172720

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAMPK alpha2 WT

   Plasmid

   #15991

   Depositor

   Morris Birnbaum

   Article

   Mu et al Mol Cell. 2001 May . 7(5):1085-94.

   Insert

   AMPK alpha2 (Prkaa2 Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6-AMPK alpha1 (1-312)

   Plasmid

   #162131

   Purpose

   Expression vector for constitutively active AMPK alpha 1

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

   Insert

   AMPK alpha1 (1-312) (PRKAA1 Rat)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUGW-VMAT2-pHluorin

   Plasmid

   #207468

   Purpose

   Lentiviral expression of VMAT2-pHluorin

   Depositor

   Robert Edwards

   Article

   Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

   Insert

   VMAT2 (Slc18a2 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2.5-THP-GFP/WGA

   Plasmid

   #80337

   Purpose

   Fluorescent Reporter for Dopaminergic Neuron Differentiation

   Depositor

   Kwang-Soo Kim

   Article

   Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

   Insert

   rat TH promoter (Th Rat)
   

   Use

   AAV

   Promoter

   rat Tyrosine hydroxylase

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Jag1-HA pIRES

   Plasmid

   #17336

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Jagged1 (Jag1 Rat)
   

   Tags

   HA X3

   Expression

   Mammalian

   Mutation

   A silent C->G mutation at 3237

   Available Since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-GLT1a

   Plasmid

   #162515

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1a (Slc1a2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. G protein alpha-s-HaloTag

    Plasmid

    #66993

    Purpose

    G protein alpha-s internally tagged with HaloTag and EE epitope

    Depositor

    Catherine Berlot

    Insert

    G-alpha-s-EE-HaloTag (Gnas Rat)
    

    Tags

    HaloTag flanked by SGGGGSGGGGS on each side was i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-Grin1 KI #2

    Plasmid

    #139658

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFPC2-CB2 SH3+

    Plasmid

    #68821

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc mTOR kinase dead

    Plasmid

    #8482

    Depositor

    David Sabatini

    Insert

    mTOR (Mtor Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D2357E V2364I

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE GFP-Syt7 KI

    Plasmid

    #131488

    Purpose

    Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid

    #105714

    Purpose

    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

    Depositor

    Rylan Larsen

    Has Service

    AAV8

    Insert

    GCaMP6s
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-EF1a-jGCaMP8m-WPRE

    Plasmid

    #176757

    Purpose

    AAV-mediated expression of GCaMP8m under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.GFAP.iGluSnFR3.v857.PDGFR

    Plasmid

    #178336

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Au1-mTOR-Wild type

    Plasmid

    #26036

    Depositor

    Fuyuhiko Tamanoi

    Article

    Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

    Insert

    mammalian target of rapamycin-wild type (Mtor Rat)
    

    Tags

    AU1

    Expression

    Mammalian

    Available Since

    Sept. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pFsynW SYT1 reporter

    Plasmid

    #197275

    Purpose

    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAMPK alpha2 K45R

    Plasmid

    #15992

    Depositor

    Morris Birnbaum

    Article

    Mu et al Mol Cell. 2001 May . 7(5):1085-94.

    Insert

    AMPK alpha2 (Prkaa2 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K45R

    Promoter

    CMV

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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