We narrowed to 4,333 results for: U6 gRNA

1. gRNA-eGFP-Reporter

   Plasmid

   #60719

   Purpose

   Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and mouse U6

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS U6 WASp RNAi

   Plasmid

   #11775

   Depositor

   Jack Strominger

   Article

   Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

   Insert

   WASp RNAi (WAS Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pBS U6 WIP RNAi

   Plasmid

   #11774

   Depositor

   Jack Strominger

   Article

   Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

   Insert

   WIP RNAi (WIPF1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. AAV-U6gRNA1-U6gRNA2-TnT-Cre

   Plasmid

   #87682

   Purpose

   AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

   Depositor

   William Pu

   Article

   Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

   Inserts

   gRNA1
   gRNA2
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   U6 and cTnT

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122089

   Purpose

   U6 driven SpCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NmCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122091

   Purpose

   U6 driven NmCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

   Plasmid

   #212961

   Purpose

   Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_backbone
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBluescript_VEGFA-TS3_SaCas9_sgRNA2

   Plasmid

   #107330

   Purpose

   U6 driven SaCas9 sgRNA expression for VEGFA site 3

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   VEGFA-TS3 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAT_g2 gRNA

   Plasmid

   #86006

   Purpose

   plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   pU6-AAT_g2 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-SaCas9-U6-sgSlc17a6

    Plasmid

    #124847

    Purpose

    Mutagenesis of Slc17a6 with SauCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 gRNA (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR/U6 HDAC4 shRNA

    Plasmid

    #32220

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    shRNA against murine HDAC4 (Hdac4 Mouse)
    

    Use

    RNAi; Gateway u6 entry vector

    Tags

    Expression

    Mutation

    Promoter

    human U6

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pENTR/U6 HDAC5 shRNA

    Plasmid

    #32222

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    shRNA against mouse HDAC5 (Hdac5 Mouse)
    

    Use

    RNAi; Gateway u6 entry vector

    Tags

    Expression

    Mutation

    Promoter

    human U6

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAdSh.U6.gRNAGFP

    Plasmid

    #58255

    Purpose

    U6 promoter-driven single guide RNA complementary to eGFP

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAGFP cassette
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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