We narrowed to 8,683 results for: sgRNA

1. pSEVA19_sgRNA-C0_M13ps

   Plasmid

   #231410

   Purpose

   M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C0
   

   Use

   Synthetic Biology

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA19_sgRNA-1_M13ps

   Plasmid

   #231409

   Purpose

   M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   sgRNA-1
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA_MET_uORF1

   Plasmid

   #216537

   Purpose

   gRNA and template for MET 5'UTR uORF1 mutation

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

   Insert

   pegRNA MET 5'UTR uORF1 mutation
   

   Expression

   Mammalian

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA_MET_uORF2

   Plasmid

   #216538

   Purpose

   gRNA and template for MET 5'UTR uORF2 mutation

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

   Insert

   pegRNA MET 5'UTR uORF2 mutation
   

   Expression

   Mammalian

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. p103DestTol2pA2-4xU6sgRNA

   Plasmid

   #227778

   Purpose

   Destination vector with U6 driven 4 sgRNAs

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Insert

   4xU6:sgRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA1_Cas9-hGem_Smc4

   Plasmid

   #217667

   Purpose

   expresses Cas9-hGem and guideRNA for tagging of SMC4

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2 NT sgRNA5

   Plasmid

   #164930

   Purpose

   Expresses Non-targeting sgRNA5 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA5 (verified for knockout)
   

   Use

   Lentiviral

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SHLD3 KO sgRNA #1

   Plasmid

   #207105

   Purpose

   pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   CGCTATCAAGATTTATACCT
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HaloTag KO sgRNA

   Plasmid

   #207102

   Purpose

   pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GTCGATGTTGGTCCGCGCGA
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SHLD3 KO sgRNA #2

    Plasmid

    #207106

    Purpose

    pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CTGAAGGAACAGACTAATTC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 53BP1 KO sgRNA

    Plasmid

    #207104

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGATTCTCAGCCTGAAAGCC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-sgRNA-hcr3

    Plasmid

    #193660

    Purpose

    Expression of tandem pre-sgRNA array hcr3 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6-sgRNA-hcr5

    Plasmid

    #193661

    Purpose

    Expression of tandem pre-sgRNA array hcr5 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-sgRNA-pcr4

    Plasmid

    #193662

    Purpose

    Expression of tandem pre-sgRNA array pcr4 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LENTICRISPR-LUC7L2_sgRNA1

    Plasmid

    #201602

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LENTICRISPR-PGM2_sgRNA2

    Plasmid

    #201617

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    PGM2 (PGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LENTICRISPR-PGM2_sgRNA1

    Plasmid

    #201616

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    PGM2 (PGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LENTICRISPR-UCK2_sgRNA2

    Plasmid

    #201633

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UCK2 (UCK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. LENTICRISPR-RPE_sgRNA2

    Plasmid

    #201619

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    RPE (RPE Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LENTICRISPR-PFKM_sgRNA1

    Plasmid

    #201610

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PFKM (PFKM Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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