We narrowed to 8,859 results for: sgRNA

Showing: 421 - 440 of 8859 results

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PB_rtTA_BsmB-HNRNPK_sgRNA_3

Plasmid

#242898

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-sgRNA006_CTCF

Plasmid

#232928

Purpose

Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9 (Ctcf S. pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA047_Podxl

Plasmid

#232932

Purpose

Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Podxl S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-1pro

Plasmid

#231151

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbSTM-5'UTR

Plasmid

#231150

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbSTM-5?UTR

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PE-pegRNA(BG)-nsgRNA(BG)

Plasmid

#232727

Purpose

A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

Depositor

David Brafman

Article

Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

Insert

pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_LacI_pLac-sgRNA-1

Plasmid

#231408

Purpose

sgRNA-1 expression from an IPTG-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
LacI

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_AraC_pBAD-sgRNA-1

Plasmid

#231407

Purpose

sgRNA-1 expression from an arabinose-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
AraC

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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