We narrowed to 9,096 results for: sgRNA
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Plasmid#75387PurposeN22p-3XRFPnlsDepositorInsertN22p
UseLentiviralTags3XmCherryExpressionMammalianPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRExpressionMammalianPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU3m
Plasmid#66193Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU3m (SpeI site destroyed)Available sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeExpressionBacterialAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA950
Plasmid#122239PurposeCROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.DepositorInsertBFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
UseCRISPR and LentiviralExpressionMammalianAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR - EGFP sgRNA 3
Plasmid#51762PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 3
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EFS-PCP-3XGFPnls
Plasmid#75385PurposePCP-3XGFPnlsDepositorInsertPCP
UseLentiviralTags3XsfGFPExpressionMammalianPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti_sgRNA(MS2)_zsGreen1
Plasmid#138463Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1DepositorPublicationInsertzsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG_GI3
Plasmid#111594PurposehU6-sgRNA GI Library vectorDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1_sgRNA
Plasmid#100554PurposeExpresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGATDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1 sgRNA
PromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
Plasmid#71485PurposeBase plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon armsDepositorTypeEmpty backbonePromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sp-sgRNA-mDNMT1_nick-lenti
Plasmid#135959PurposeS. pyogenes nicking sgRNA for mouse DNMT1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmDNMT1_nick
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA
Plasmid#133348Purposeexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)DepositorInsertsgRNA_blaA and sgRNA_cpaA
UseCRISPRPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX-EGFP-g1
Plasmid#107273PurposeeGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP sgRNA-1
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU6a/LacZ
Plasmid#66195Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6aAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SUPERBLADE5
Plasmid#134913PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-AtU3d/LacZ
Plasmid#66201Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterAtU3dAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA1-Tat
Plasmid#138478PurposeExpresses HIV Tat for efficient sgRNA packaging.DepositorInsertTat
ExpressionMammalianAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by