Addgene

Showing: 421 - 440 of 52883 results

Results per page:

Sort results by:

pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S400D

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 G399S

Plasmid

#16158

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

G399S

Promoter

T7

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pJM601-TRA-2::FLAG

Plasmid

#27129

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 FLAG (tra-2 Nematode)

Use

Tags

3x FLAG

Expression

Worm

Mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and…

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2TK-PDZ(HtrA2)

Plasmid

#16207

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

Insert

HtrA2 (HTRA2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 350–459

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S142A

Plasmid

#16153

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S142A

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmTral

Plasmid

#21721

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2(neutral2-6)-mCherry

Plasmid

#104181

Purpose

Expresses fusion of CRY2(neutral aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(neutral2-6)-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2PHR K2S M3S D4G K5S K6S

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDOE11 mas:mTq2 transient

Plasmid

#124248

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-delta133HtrA2 S142D

Plasmid

#16154

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S142D

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
STRADB gRNA (BRDN0001146450)

Plasmid

#75494

Purpose

3rd generation lentiviral gRNA plasmid targeting human STRADB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STRADB (STRADB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Opie2-PuroR[traF]

Plasmid

#131615

Purpose

Expresses a functional PuroR only in females and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Insert

PuroR[traF]

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-delta133HtrA2 S400A

Plasmid

#16155

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S400A

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE intramol-1m

Plasmid

#13551

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207W (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

A207W, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL c2x Tral FL

Plasmid

#113012

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral WT

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM614-TRA-2::GFP

Plasmid

#27128

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 GFP (tra-2 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

replaced stop codon with AgeI and EcoRI added GFP…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAPTrap-LacZ-IRESMuro

Plasmid

#82507

Purpose

Vector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

LacZ-IRESMuro

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASt- Crb intraGFP

Plasmid

#67525

Purpose

Expression of transmembrane and the intracellular domains of Crb with GFP.

Depositor

Elisabeth Knust, Marta Llimargas Casanova

Insert

crumbs (crb Fly)

Use

Drosophila

Tags

GFP

Expression

Insect

Mutation

aa97-2103 deleted (extracellular domain)

Promoter

hsp70

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pWB-TRAF2 S11A

Plasmid

#44134

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Serine 11 to Alanine

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Use

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge