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We narrowed to 11,427 results for: Vars

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Showing: 4381 - 4400 of 11427 results

  8. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  11. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

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  12. pAM4891 (pCV0003)

    Plasmid
    #120080
    Purpose
    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.
    Depositor
    Susan Golden
    Article
    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku
    Insert
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    Use
    Other
    Mutation
    mobA Y25F
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120080-plasmid-map-sequence-id-234472

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  19. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

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  20. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200403
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200403-plasmid-map-sequence-id-396808

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Showing: 4381 - 4400 of 11427 results