We narrowed to 9,678 results for: otos

1. pKanCMV-CX4.3-7aa-mClover3

   Plasmid

   #74260

   Purpose

   Mammalian expression of C-terminal fusion of mClover3 to CX43

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   Connexin 43
   

   Use

   Tags

   mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSin-5HT-1.0

   Plasmid

   #172882

   Purpose

   make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

   Depositor

   J. Julius Zhu

   Article

   Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

   Insert

   5HT SENSOR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   sp6

   Available since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-SYN-GFP-BGHpA

   Plasmid

   #190229

   Purpose

   Expresses EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

   Plasmid

   #199352

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199364

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TB:7xSMAD_RE:MiniP

   Plasmid

   #133879

   Purpose

   7 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   7 copies the SMAD DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

   Plasmid

   #195853

   Purpose

   Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

   Depositor

   Andrew Leifer

   Article

   Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

   Insert

   Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   lim-4

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GalT-moxDendra2

   Plasmid

   #89789

   Purpose

   mammalian expression of GC localized moxDendra2

   Depositor

   Erik Snapp

   Article

   Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

   Insert

   GalT signal anchor transmembrane domain (B4GALT1 Human)
   

   Use

   Tags

   moxDendra2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-17b_Unc104-KIF5B-HALO

   Plasmid

   #169996

   Purpose

   Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

   Depositor

   Ron Vale

   Article

   Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

   Insert

   Kinesin-like protein Unc-104 fused with KIF5B
   

   Use

   Tags

   HALO-tag, 6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC-SARS-CoV-2 Orf6

    Plasmid

    #158374

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf6 (ORF6 )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209660

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    GFAP

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Use

    Tags

    Hexa his

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2ADW-lox-mCherry-hyBRET-ERK-NLS

    Plasmid

    #170290

    Purpose

    For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    lox-mCherry-lox-hyBRET-ERK-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MAC-SARS-CoV-2 E

    Plasmid

    #158368

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 E (E )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3-myc3-CUL4A delta N52

    Plasmid

    #19952

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CUL4A (cullin 4A) (CUL4A Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Missing 52 residues from N terminal CUL4A.

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pSIREN-RetroQ-shLuc

    Plasmid

    #73666

    Purpose

    pSRIEN-RetroQ vector containing control sequence luciferase

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. MAC-SARS-CoV-2 NSP1

    Plasmid

    #158352

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP1 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDF0039 DisCas7-11 only expression with CRISPR array

    Plasmid

    #172500

    Purpose

    Encodes the DisCas7-11 protein only for bacterial expression

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 only expression with CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-CVm-PTEN

    Plasmid

    #168032

    Purpose

    Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-PTEN(M265-N329)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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