We narrowed to 17,255 results for: tes
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Plasmid#55776PurposeThis G protein alpha-o construct contains internal insertions of YFP and the EE epitopeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
TagsEE epitope was introduced internally with D167E a…ExpressionMammalianMutationA BglII site was removed from the 5' untrans…PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-6xHis-sfGFP
Plasmid#199171Purposeused to express a superfolder GFP in E. coli for comparison to supercharged variantsDepositorInsertsuperfolder GFP
TagsMGHHHHHHGGAPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2 gRNA concatmer
Plasmid#84879PurposeEmpty concatmer vector in which 2 gRNAs can be insertedDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL
Plasmid#111389PurposeAAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsChETA-mRuby2
W3SL
UseAAVTagsmRuby2ExpressionMammalianMutationW3SL is built from a shortened WPRE, an upstream …PromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G protein alpha-s-HaloTag
Plasmid#66993PurposeG protein alpha-s internally tagged with HaloTag and EE epitopeDepositorInsertG-alpha-s-EE-HaloTag (Gnas Rat)
TagsHaloTag flanked by SGGGGSGGGGS on each side was i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-L
Plasmid#120160PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertType I procollagen α2 chain (Col1a2 Mouse)
TagseGFPExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TDAG51
Plasmid#32699DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman tdag51 (PHLDA1 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-mEGFP-PKCa-C1
Plasmid#112264PurposeITRACKalpha Donor ConstructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertprotein kinase C alpha (PRKCA Bovine)
TagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HSF1Δ∼PRD
Plasmid#71725Purposeplasmid expressing mic-his tag phosphorylation-deficient hHSF1DepositorInsertphosphorylation-deficient HSF1 (HSF1 Human)
Tagsmac 6xHISExpressionMammalianMutation15 phosphorylation sites in RD mutated to AlanineAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TSC2-3A (S1337A/S1341A/S1345A)
Plasmid#29360DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTSC2 (Tsc2 Rat)
ExpressionMammalianMutationS1337A/S1341A/S1345A (equivalent to S1381/S1385/S…Available sinceJan. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-mRFP-gsk3 beta S9A
Plasmid#24521DepositorInsertGSK3beta(S9A) (GSK3B Human)
UseAdenoviralTagsmRFPExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…Available sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1hLCAD_FLAG
Plasmid#24491DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLong chain acyl-CoA dehydrogenase (ACADL Human)
TagsFLAGExpressionMammalianAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMycMyt1-7zf/IRES-Red
Plasmid#22652DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyelin Transcription Factor 1 (Myt1 Mouse)
TagsMycExpressionMammalianAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-mCherry-CRY2-NLS
Plasmid#145259PurposeMammalian Expression of Nuclear Control OptoConstruct with SV40 NLSDepositorTypeEmpty backboneExpressionMammalianAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pARv-RFP
Plasmid#60021PurposepARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.DepositorTypeEmpty backboneExpressionMammalianAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hMLKL-Venus-Mito
Plasmid#106080Purposeexpression in mammalian cellsDepositorInserthMLKL-Venus-Mito (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsmitochondrial-targetting domain of Bcl-xlExpressionMammalianPromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-140)-2xFv-flag
Plasmid#106074Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-140)-2xFv-flag (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsflagExpressionMammalianMutationamino acids 1-140PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Twitch2B.WPRE.SV40
Plasmid#100039PurposeAAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorInsertTwitch2B
UseAAVExpressionMammalianPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCherryC1 VAP-A
Plasmid#226407PurposeExpression of human VAP-A fused to mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAP-A (VAPA Human)
TagsT7-Xpress tag and mCherryExpressionMammalianAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Human, Synthetic)
ExpressionMammalianAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only