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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
LifeAct-mAvicFP1

Plasmid

#129512

Purpose

Expresses LifeAct-mAvicFP1 in mammalian cells

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

LifeAct-mAvicFP1

Use

Tags

Expression

Mammalian

Mutation

mAvicFP1 is AvicFP1 F64L + A206K

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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phFluc-WT

Plasmid

#177714

Purpose

Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

Depositor

Erik Böttger

Article

Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

Insert

hFluc WT

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PBLO CasX-gRNA

Plasmid

#126419

Purpose

E. coli vector for CasX gRNA --- Used for In Vitro Transcription

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

casX gRNA

Use

CRISPR; In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-myc

Plasmid

#109422

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope

Use

Tags

BAP, HA, His6, T7, and myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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KK501: pMVP (L3-L2) FKBP DD + polyA

Plasmid

#121789

Purpose

pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

FKBP Degron Domain + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

Plasmid

#75129

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-hPGK-mCherry-SynaptoZip

Plasmid

#177317

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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SPLINTR BFP v1 backbone

Plasmid

#179779

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CB6 FFLuc-miR122

Plasmid

#35656

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

pri-miR-122 (Mir122 )

Use

AAV

Tags

Expression

Mutation

Promoter

CB

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pOPINK-VCPIP (OTU, aa 25-561)

Plasmid

#61583

Purpose

Expresses human VCPIP (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

VCPIP (VCPIP1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-24 and aa 562-1222.

Promoter

T7

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-HA-GFP11-KDEL

Plasmid

#177722

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Use

Tags

GFP11, HA, KDEL, and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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JN202: pMVP/SB/Blast-DEST

Plasmid

#121864

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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NG0750 pCI-TPI-PTC48-4H

Plasmid

#65803

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 48

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

Plasmid

#213826

Purpose

Expresses human PRDM6 with a C-terminal V5 epitope tag.

Depositor

Bjoern Schwer

Article

Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

Insert

PRDM6 (PRDM6 Human)

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN5

Plasmid

#110639

Purpose

Retroviral expression of Flag-SFXN5 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN5 (SFXN5 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67295

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-10xMS2 oligo-library based

Plasmid

#158200

Purpose

Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

10 binding sites of MS2 from oligo library experiment

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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